雌激素的即时动态检测是绝经期女性安全使用激素替代治疗、实现个体化用药预警风险事件的重要临床需求。目前基于抗原抗体原理的方法可实现雌激素体外定量检测，却无法实现在体动态即时检测。本项目拟采用D-/L-异核苷/2’-OMe/2’-F/磷硫代等修饰方法，提高雌激素核酸适配体的靶标特异性结合力和血液中的稳定性；结合适配体开关结构，构建电化学微针检测为载体的雌激素体内检测新型传感器，实现血液中雌激素动态检测，并利用去势动物模型和人源真实血液样品开展应用测试及传感器参数优化的基础性研究，为未来个体化的激素替代诊疗提供定量动态检测的新方法和技术基础。

The real-time monitoring of estradiol in situ in the living body would greatly advance the development of personalized medicine.in menopausal women using hormone therapy and avoid the risk for breast cancer, to achieve this, Aptamer instead of traditional antibody is used . Firstly, we perform D-isonucleotide/2'-OMe or 2'-F-nucleotide and phosphorathiote-alkylated to modified estradiol aptamer with which facility its stability in blood stream. Secondly, an electrochemical aptamer-based biosensors, a versatile sensing platform that supports the continuous, real-time measurement of specific molecular analytes is established. Then sensor will be used to measure in vivo concentrations of estradiol in human blood and in living ovariectomized rat model. The novel methods and technical sensor could be a powerful enabler for personalized medicine by ensuring delivery of optimal drug doses for individual patients based on direct detection of physiological estradiol parameters.