Th9细胞（分泌IL-9的CD4+ Th细胞）是新近发现的Th细胞亚群，早先认为IL-9在Th2细胞介导的抗寄生虫免疫应答和变应性免疫应答发挥重要作用，现发现IL-9主要由Th9而非Th2细胞分泌，这就需要对原本归于Th2类的生理或病理改变的机制进行重新探讨；Nuocytes是一类新发现的依赖于IL-25和IL-33的non-B/non-T细胞，在Ⅱ型免疫应答的早期发挥重要作用。本研究以Th9细胞和Nuocytes作为研究对象，采用哮喘小鼠作为动物模型，首先观察Th9细胞和Nuocytes在哮喘小鼠体内的分布，研究Th9细胞和Nuocytes在哮喘小鼠模型诱导过程中的作用，并通过抗体或siRNA封闭IL-9以寻找治疗靶点。其次寻找Nuocytes在促进Th9细胞分化过程中的关键分子，通过体内外实验阐明Nuocytes对Th9细胞分化的作用及分子机制，为进一步了解哮喘病的发病机制提供新思路。

Th9 cells (which secretes IL-9 of CD4 + Th cells) is a newly identified Th cell subset. IL-9 was previously thought to play an important role in Th2 cell-mediated anti-parasite immune response and allergic immune responses. However, it is now found to be mainly secreted by the Th9 cells rather than Th2 cells. Thus, the mechanisms which were originally attributed to a Th2 type of physiological or pathological changes needs to be re-investigated. Nuocytes are newly discovered cells dependent on IL-25 and IL-33 and are non B or T cells that plays an important role in the early type Ⅱ immune responses. The objective of the study was to investigate the role of Th9 cells and Nuocytes in asthma mice model. We first assessed Th9 cells and Nuocytes distribution in asthma mice and then we also used siRNA and IL-9 antibodies for blocking in order to find therapeutic targets. Secondly, we also tried to find key elements for Nuocytes in promoting Th9 cell differentiation. Further, in vitro and in vivo experiments were used to clarify the role and molecular mechanism for Nuocytes in Th9 cell differentiation. This will provide new ideas for further understanding of the pathogenesis of asthma.