本项目主要研究利用纳米磁性粒子-贝毒抗体-螯合剂二乙三胺五乙酸-稀土离子方法和Fe2O3@Au磁性纳米核壳粒子-贝毒抗体方法实现对贝毒分子进行金属离子标记。然后利用所建立的贝毒分子金属标记技术、结合毛细管电泳-电感耦合等离子体质谱联用技术，建立一种分析检测近海海域和海产品中痕量贝毒的新方法，以解决近海海域和海产品中痕量贝毒快速检测的技术难题。本研究所建立的方法充分利用了纳米磁性粒子易于收集和抗体具有优异特异性的特点，同时结合了毛细管电泳分离速度快、分离效果好以及ICP-MS对于金属元素检测的高灵敏度、高稳定性和强抗干扰能力的优点。所建立的方法具有灵敏度高、稳定性好、抗基质干扰能力强和简单快速等显著特点，可望用于海水和海产品实际样品中痕量贝毒的快速和高灵敏检测。利用所建立的分析方法，同时对福建近海海域和海产品中的贝毒污染情况进行调查，为福建近海海域贝毒污染的控制提供理论依据和技术支撑。

The main aim of this study is that to develop a novel method for the detection of trace saxitoxins in the coastal seawater and seafood based on capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) together with magnetic nanoparticles-antibody-DTPAA-lanthanide labeling or Fe2O3@Au core-shell nanoparticles-antibody labeling, to resolve the technical problems of sensitively and fast determining trace saxitoxins in coastal ocean and seafood. The analytical method developed in this study combined the merits of magnetic nanoparticle, antibody, capillary electrophoresis and ICP-MS, has obviuos analytical advantage such as high sensitivity, better stability, robust resistance to the complex matrix, simple operation and short analysis time. The novel method developed in this study can be used to determine trace saxitoxins in real coastal seawater and seafood samples with a satisfied accuracy and stability. In addition, with the help of the developed method, the saxitoxins in the real seawater and seafood collected from coastal ovean of Fujian province will by determined in detail in order to provide the database and technique support for controlling the pollution of red tide toxin in coastal ocean of Fujian province.