本课题的主要目标是建立可吸入颗粒物中主要毒性金属元素的形态分析方法，并利用所建立的方法详细研究不同起源气溶胶中各金属元素的化学形态特征,探索发现气溶胶中金属离子的化学形态和气溶胶起源之间的关联，为建立一种新的基于气溶胶中各金属离子的化学形态特征的追踪气溶胶起源的方法提供理论依据。同时，结合质粒DNA评价法和流式细胞分析法分析评价含不同化学形态金属元素气溶胶的毒性，解明气溶胶中特定金属离子的化学形态对气溶胶毒性和气溶胶中主要有机毒性物质降解反应的影响，为分析气溶胶的毒性来源、正确评价不同起源气溶胶的毒性以及其对大气环境和人体健康的影响、分析探讨发生在气溶胶表面的多相反应的机理提供新的基础数据和理论依据。

The main aims of this study is that to develop a sensitive analytical method for the speciation analysis of each toxic metallic element in the fine aerosols and further to investigate the chemical speciation characteristics of each toxic metallic element in different aerosols, which originating from different sources, in detail by using the developed method. Based on above experiments, we hope to find the relationship between the chemical speciation characteristics of each toxic metallic element and the source of aerosol, and establish a new method of tracking aerosol's sources, which based on the chemical speciation characteristics of various metal ions in the aerosols. In the mean time, by evaluating the toxicity of the different aerosols, which has different speciation characteristics of toxic metallic elements, with plasmid DNA assay and annexin V-FITC/PI flow cytometric method, we hope to realize the effect of chemical speciation characteristics of various metal ions on the toxicity of aerosols and the degradation of organic components in the aerosols, in order to provide novel database and theory for the discussion of the toxic source of aerosols, more accurate evaluation of the toxicity of different aerosols, more accurately assessing the impact of different aerosols on the atmospheric environment and people's health, and discussing the mechanism of heterogeneous reactions happened on the surface of aerosols.