磺胺类抗生素（SAs）被广泛用于畜牧和水产养殖。SAs具有良好的持久性，所以易在环境、畜禽肉类和水产品中残留，进而对人体健康产生伤害。SAs种类繁多，为确定或筛查环境、畜禽肉类和水产品中SAs的残留，需要对常用的所有种类的SAs进行分析检测，因此建立一种可以同时快速检测多种SAs的高通量、低成本的分析方法具有重要意义。本项目以18种常用SAs为目标，根据SAs的取代基结构特征选取代表性靶标分子，采用“并联-交替”的捕获-指数富集配体系统进化技术筛选对18种常用SAs都具有识别和结合能力的SAs广谱特异性核酸适配体。并以所筛选的SAs广谱特异性适配体为探针、结合磁性粒子、无酶核酸放大技术和纳米过氧化酶模拟物，建立灵敏度高、选择性好、抗干扰能力强和稳定性好的可同时快速检测18种常用SAs的可视化检测新方法，以期为环境、畜禽肉类和水产品中SAs残留的现场快速检测和筛查提供一可靠和低成本的新技术。

Sulfonamide antibiotics (SAs) have been widely used in animal agriculture and aquaculture. SAs are easy to be remained in the environment, poultry meats and aquatic products since their good persistence, and thus harm human’s health. It was well known, SAs contained a lot of individuals, which has a similar chemical structure. Therefore, it is necessary to simultaneously analyze all kinds of SAs in order to confirm or screen the SAs residues in environment, poultry meat and aquatic products. So, it is of great significance to establish a high-throughput visual method for the simultaneous detection of multiple SAs. In this project, we aimed 18 kinds of commonly used SAs, and selected the representative target molecules according to the structural characteristics of the substituents of SAs. Then, the SAs class-specific DNA aptamer, which has excellent specificity and binding ability to above 18 SAs, was selected by using “parallel-and-serial capture-SELEX” method. Additionally, a novel visual method will be developed for the simultaneous and rapid detection of above 18 SAs with high sensitivity, excellent selectivity, strong anti-interference ability and good stability based on the selected SAs class-specific DNA aptamer together with magnetic beads, enzyme-free DNA amplification technique and nano-materials peroxidase mimetic, in hope of providing a reliable and cost-effective technique for the on-site and rapid detection and screening of SAs residues in environment, poultry meat and aquatic products.