葡萄芪合成酶基因的表达产物白藜芦醇不仅增强植物抗病性，而且存在于葡萄浆果及其制品中，对人体具有抗氧化、抗衰老和防癌的作用。本项目是在前期获得中国野生葡萄高表达的芪合成酶基因及其在成熟果实中表达高于其他器官组织的基础上，拟开展：1）白粉菌胁迫诱导高表达的芪合成酶基因及其转录因子表达，进行高表达芪合成酶基因葡萄组织的转录组测序研究；2）遴选获得高表达芪合成酶基因基因与转录因子基因，探究芪合成酶基因调控机理；3）对这些基因在葡萄器官组织和亚细胞水平定位与功能表达研究；4）进一步研究胁迫条件下芪合成酶及其产物白藜芦醇在合成、转运、积累、作用和降解的动态变化规律，阐明高表达的芪合成酶基因功能；5）利用转基因技术，向欧洲葡萄品种中转入所获基因，创造葡萄新种质。本研究将为弄清芪合成酶基因功能与作用机制，创造抗病优质葡萄新种质提供基因资源与科学依据。

Resveratrol enriched in grape berries and its products, not only enhances the resistance to fungal pathogens but also plays important roles in human health, including anti-cancer, antioxidant, and reduction of cardiovascular disease. Stilbene synthase (STS) is a key enzyme in the biosynthesis of resveratrol. This project is based on our previous research, which has verified that the expression of stilbene synthase gene in the berry was significantly higher than other organs/tissues from most Chinese wild Vitis species. The main projects are carried out as following:.1) Transcriptome analysis of high expression of stilbene synthase genes and their transcription factors was carried out in the organs of Chinese wild grapevine induced by Uncinula necator. .2) Explore regulations and expressions of selecting high expression of stilbene synthase genes and their transcription factors from the organs. .3) Localizations both of organization and subcellular and functional analysis of these favorable genes in transgenic plants and protoplasts. .4) Dynamic observation of the synthesis, transport, accumulation and degradation of STS and their expression resveratrol under stress by laser scanning confocal microscope to better understand the function of stilbene synthase genes;.5) Agrobacterium-mediated transformation and over-expression of the interesting genes from Chinese wild grape species into Vitis vinifera through somatic embryogenesis system to generate the highly accumulation of resveratrol and novel disease-resistance grapevine germplasm..The research focuses on the regulatory mechanism of stilbene synthase genes in response to biotic and abiotic stress as well as their function to defense powdery mildew. Additonnally, this study also provides gene resource for disease-resistance breeding and a base for future scientific research.