JH-Met信号调控大猿叶虫卵黄发生关键基因转录的机制研究

昆虫卵黄物质积累对早期胚胎发育至关重要。保幼激素(JH)诱导卵黄发生，但具体调控机理并不明确，主要原因在于JH信号途径中参与卵黄发生的关键分子尚未得到鉴定。因此，筛选卵黄发生中JH直接效应基因并解析其转录调控机制，是揭示昆虫卵黄发生分子机制的核心工作。申请人前期研究发现，注射JH类似物(JHA)可以显著诱导大猿叶虫Colaphellus bowringi滞育型个体卵黄发生。本项目拟以JHA诱导的大猿叶虫卵黄发生虫体模型为材料，通过数字基因表达谱分析，筛选JH功能受体Met调控下的直接效应基因。通过启动子激活分析和RNAi实验，揭示JH-Met信号直接效应基因的转录激活机制及其在卵黄发生中的关键作用。研究结果不仅可阐明JH-Met信号调控大猿叶虫卵黄发生的分子机制，而且有助于揭示昆虫生殖机制和JH信号转导通路，还能为害虫防治提供新靶标和新思路。

The accumulation of yolk is essential for insects early embryonic development. Juvenile hormone (JH) regulates vitellogenesis, but the signal transduction pathway is unclear. The primary reason is that the JH-targeted key genes involved in vitellogenesis have not been identified. Given that, screening the JH-targeted genes and determining the transcription regulatory mechanisms are the most important work. Our previous studies have revealed that JH analog (JHA) methoprene injection significantly promoted vitellogenesis of diapause-destined cabbage beetle Colaphellus bowringi. In regard of that, we plan to screen vitellogenesis-related genes controlled by JH functional receptor Methoprene-tolerant (Met) using Digital Gene Expression Profiling analysis based on the JHA-induced vitellogenesis individuals. RNA interference will be used to verify the function of the key genes on vitellogenesis regulation. Then, The transcriptional activation of vitellogenesis key genes controlled by JH-Met signaling will be analyzed via promoter activation assay. Finally, This study will reveal the mechanism by which JH-Met signaling regulates vitellogenesis of C. bowringi, deepen the mechanism of insect reproduction and JH signaling pathway, and then provide new ideas for pest control.