电子束辐照场对花生粕中黄曲霉毒素 B1 消减机制研究

我国花生粕年产400万吨，因黄曲霉毒素（AFT）超标严重影响其充分利用，针对现有消减方法缺陷、规模化应用需求及花生粕非均质固态特性等现状，寻找安全有效又不影响花生粕品质的消减AFT方法亟待解决。申请人前期研究已发现5 kGy电子束辐照场（EBI）可穿透花生粕消减其中67.96%AFB1，同时证实EBI在剂量范围内对花生粕品质无影响且有规模化应用潜质，但降解机理尚不明确，这成为该法消减花生粕中AFT应用的瓶颈。本研究以AFB1为目标物，研究EBI作用下AFB1降解行为及动力学；采用超高效液相色谱-四级杆飞行时间质谱与高效液相色谱-核磁共振联用技术，筛查AFB1各级降解产物，追踪降解物动态变化，阐明AFB1的降解路径，结合量子化学从能量角度诠释电子束辐照场消减AFB1机理；采用体外AFB1细胞毒理模型评估降解产物的安全性，为EBI在食品行业的应用提供理论指导和基础数据。

Aflatoxins (AFT) are highly toxic, mutagenic, teratogenic and carcinogenic compounds. Although 4 million ton peanut meals are produced every year in our country, it could not be utilized sufficiently because of the high quantity of AFT. Thus, a safe and effective detoxification method in peanut meal is needed to find at present situation, which are the short of present detoxification method, the need to scale applications, and the heterogeneous nature of peanut meal. It is found that 67.96% AFB1 can be deleted by 5 kGy electron beam irradiation (EBI), and at the same time the quantity of the peanut meal is not affected by it. The most important is that the detoxification method can be used in industrial. But the mechanism of EBI to remove AFB1 remains unknown, which become the development bottleneck of EBI in food safety..AFB1 in peanut meal is the target in this study, and the degradation behavior & degradation kinetics will be studied firstly. Secondly, the degradation products will be screened and identified by UPLC-Q-TOF/MS and HPLC-NMR. And then, the kinetic variation of the degradation products under different condition will be tracked and the degradation pathway will be proposed at the same time. At last, the degradation mechanism of AFB1 in peanut meal using EBI will be elucidated from the perspective of quantum chemistry, and the cytotoxicity of the degradation products will be evaluated by in vitro evaluating model. Theoretical direction and basis information will be supplied for EBI to be applied in food widespread.