周围神经毁损伤的修复是医学难题。临床实践中供体神经来源不足影响着周围神经损伤的修复。前期研究发现可以利用周围神经的出芽再生能力（倍数再生）修复严重的周围神经损伤（放大修复）。但受传统方法的限制，放大代偿修复的研究中末梢效应器的结构重塑与功能恢复之间的关系研究不足，是影响重建方案选择和疗效的瓶颈。本项目将在前期研究的基础上，利用先进的组织光透明和整体成像技术，结合多重示踪以及跨突触标记方法，探讨放大代偿修复过程中，末梢效应器的整体结构重塑与功能恢复的关系：1、放大代偿修复过程中不同放大比例对末梢效应器功能恢复的影响；2、放大代偿修复后运动终板的重塑模式；3、放大代偿修复过程中供体神经脊髓运动神经元的变化规律。本项目有望从神经解剖学及功能学的角度，阐释周围神经放大代偿修复后末梢效应器结构基础与功能恢复的关系，为周围神经损伤放大代偿修复的方案设计提供策略依据，具有重要的科学意义和临床价值。

The repair of the peripheral nerve injury is a medical challenge. Insufficient donor neural sources in clinical practice affect the repair of peripheral nerve injury. Previous studies have found that the peripheral nerve regeneration ability (multiple regeneration) can be used to repair severe peripheral nerve injury (magnification repair). However, limited by the traditional methods, the relationship between structural remodeling and functional recovery of peripheral effectors in the study of magnifying compensation repair is insufficiently delineated, which is the bottleneck affecting the choice and efficacy of reconstruction treatments. Based on the previous research, this project will use advanced optical clearing technique and overall imaging technology, combined with multiple labeling and trans-synaptic tracing methods to explore the relationship of overall structural remodeling and function of the peripheral effector during the process of magnifying repair: 1. The effect of different magnification ratios on the recovery of peripheral effector function in the process of magnification compensation; 2. The remodeling mode of the endplate after magnification repair; 3. The variation of spinal motoneurons corresponding to donor nerve during the repairing. This project is expected to explain the relationship between the structural basis and functional recovery of peripheral effectors after peripheral nerve amplification and compensation from the perspective of neuroanatomy and neural function, and provide a strategic basis for the repairing method of peripheral nerve injury.