阔叶十大功劳是小檗科十大功劳属重要的药用植物，其所含的原小檗碱型生物碱具有多种生物活性。然而其主要药用部位根茎（功劳木）被采集后，叶片常作为废弃物处理，这造成了药用资源的极大浪费。我们前期研究表明，紫外光联合暗培养胁迫（活性酶诱导）下阔叶十大功劳叶片中生物碱含量大幅提高，基于双向电泳技术的差异蛋白质组学研究发现，活性酶诱导促使叶片中的腺苷甲硫氨酸合酶的表达上调可能是影响其生物碱合成的因素之一。.本项目拟采用组合肽配体库技术对阔叶十大功劳叶片中生物碱合成相关的低丰度蛋白进行富集，结合灵敏度更高的label-free蛋白质组学技术，揭示阔叶十大功劳叶片中生物碱合成途径中关键蛋白的表达变化；并应用荧光定量PCR技术对相关基因的表达量进行分析。从蛋白水平诠释阔叶十大功劳叶片响应活性酶诱导的机制，该研究不仅为药用植物中低丰度蛋白的研究提供了新的方法，也为阐明中药活性成分生物合成途径提供新的技术。

Mahonia bealei is an important medicinal plant of the family Berberidaceae. It contained many active benzylisoquinoline alkaloids. However, the roots and stems of M. bealei (Gonglaomu) are collected as main medicinal parts and the leaves are abandoned, which caused the waste of medicinal resources. Our previous studies showed that the content of alkaloids in M. bealei leaves under UV and dark stresses (active-enzyme induction) was significantly increased. The result of proteomics based on 2-DE showed that the abundance of S-adenosyl-L-methionine synthetase in M. bealei was increased after active-enzyme induction, which might be responsible for the enhanced biosynthesis of alkaloids..In the present study, to explore the mechanism of active-enzyme induction in M. bealei, a gel-free/label-free proteomic technique was used in combination with combinatorial peptide ligand libraries (CPLL). CPLL technique is applied to enrich the low-abundance proteins, which will be beneficial to research the biosynthesis pathway of alkaloids in M. bealei leaves. And the expression of genes related to the biosynthesis of alkaloids under active-enzyme induction in M. bealei was analyzed by qRT-PCR technique. The present study provides not only a new method for low-abundance proteins in medicinal plants, but also a new technique to reveal the biosynthesis pathway of active ingredients in Traditional Chinese Medicine.