骨细胞在体外的细胞培养矿化是研究生物矿化调控分子机制的基本手段。本研究基于新型的plastic compression技术构建仿osteoid结构三维微纳米有序胶原模板，以MC3T3-E1成骨细胞为模型细胞，开展BMPs和周期应力协同作用下在仿osteoid结构胶原模板上细胞的生物矿化行为的研究，实现细胞培养中"生物合成"矿化胶原纤维构建的类骨组织结构，并基于BMPs/Smad通道分析成骨细胞生物矿化的调控分子机制。本研究最重要的特色之处就在于仿osteoid结构的构建，将细胞生物矿化的研究提升至三维有序仿生模板上。本研究不仅探讨硬组织形成和再生的本质所在，为实现骨组织工程化和重建提供重要理论；同时可为病理性矿化的研究提供研究方法和相关机理。

Bone cell culture system are essential tools for the investigation of the moluecular mechanisms that regulate biomineralization. The study focuses on the biomineralization behavior of MC3T3-E1 cells in the 3-dimension tempalet with nano- and micro organized sturcture collagen templates under the regulate of BMPs and mechanical stimulation . The templates with osteoid-like structure are to fabricated by plastic compression. A bone-like tissue with mineralized collagen is to be "biosynthesised" in the study. On the pathway of BMPs/SMads, the molecular mechnisams of regulate biomineralization are going to be analysized during the co-culture of the osteoid-like template and MC3T3-E1 cells . The charicteristic feature of the study lies in the construction osteoid-like structure, which provide an biomimetic envirnment to regulate the mineralization of osteoblast. Data on mineralization mechanisms obtained from cell culture models are valuable for the rational design of bone repair strategies and tissue engineering constructs, and biomaterial innovation. Such studies also provide fundamental information on biomineralization important to understanding basic skeletal (and dental) biology, and to managing mineralization-related pathologies such as the osteomalacias and ectopic calcifications.