随着手性化学品的广泛使用和重金属污染事件频发，手性污染物-重金属的共存成为一个普遍现象。然而，手性污染物与重金属之间的对映体选择性交互作用会影响到彼此在环境中的存在形态，进而影响到其生物可利用性和生态毒性。因此，本项目拟以三种手性污染物和铅、铜、银等典型重金属污染物为研究对象，建立手性污染物的对映体分析方法，探讨手性污染物与重金属配合物的结构、功能，揭示手性污染物对重金属离子价态和赋存形态的对映体选择性影响规律；选用拟南芥和淡水绿藻为模式生物，从形态、组织和细胞水平研究手性污染物对重金属毒性的对映体选择性效应，阐明模式生物体内手性污染物和重金属形态、分布特征、抗氧化系统响应与毒性效应之间的内在联系，揭示手性污染物对重金属生态毒性对映体选择性影响的分子机制，为此类复合环境污染物的环境安全评价提供科学依据。

With the widespread application of chiral organic chemicals and the increasing occurrence of heavy metal contamination, the co-contamination of chiral organic chemicals and heavy metals has become a common phenomenon in the water environments. However, the interactions between heavy metals and chiral organic contaminants can result in speciation changes of heavy metals. It is therefore an open question as to whether chiral organic contaminants exist in contaminated aquatic environments to act as a selective agent to mediate the toxicity of heavy metals. To decipher the puzzle, the aim of the present work was to investigate the interaction between heavy metal and chiral organic contaminant, the impacts of this interaction on the toxicity of heavy metals in the aquatic environments. Given this background, Arabidopsis thaliana and Scenedesmus obliquus are chosen as representative biomarkers. Three kinds of chiral pollutants and Pb, Cu, Ag are chosen as our model chiral organic contaminants and heavy metal contaminants. Attempts were made to explore the mechanisms involved by examining the changes of growth inhibition, chlorophyll fluorescence of plant cells, catalase activity, the species and distribution of heavy metals in plants using a combination of radiation technique combined with conventional chemical analysis methods and ecological toxicity evaluation method. This study for the first time points to the important impact of interactions between heavy metals and chiral organic contaminants on toxicity of heavy metals and provides direct microscopic and spectral evidence for the mechanisms involved, which should be considered in future risk assessment.