针对植物源抗菌剂EGCG（表没食子儿茶素没食子酸酯）在食品、化工等领域应用中存在的抗菌机理不明晰、化学性质不稳定（易氧化）等突出问题，基于申请人2018年2月发表论文中对“EGCG抗菌效应与Cpx诱导产生的ROS相关”的前期研究，项目拟对蛋白水解酶Ftsh、DegP的基因进行敲除和过表达，通过检测CpxP的转录水平、ROS的含量以及菌株存活率，从Cpx信号通路水平深入阐释EGCG的抗菌机理。同时为克服EGCG易氧化的问题，合成低廉、环保、活性利用度高的EGCG/纤维素聚合物新型水凝胶复合物。这将为开发中国传统特色农产品（茶叶）资源、环境友好地利用自然界丰富的纤维素原料提供理论依据和应用指导；并对保证食品安全，保障国民健康具有重要意义。

As an antibacterial agent derived from plant, EGCG has been widely used in many fields including food preservation and chemistry engineering. However, since its antibacterial mechanism still remains unclear and it is very easy to be oxidized, the application of EGCG is limited. Based on our preliminary work that the antibacterial activity of EGCG is triggered by ROS induced via Cpx system（published on Feb 2018）,in this project, to further study the antibacterial mechanism related to the Cpx pathway, we plan to construct DegP and Ftsh gene deletion and overexpression mutant strains and determine the CpxP transcription level, ROS level and the survival rate in these mutants. Besides, to overcome its instable chemical property, we propose to obtain a novel hydrogel complex containing EGCG and cellulose ploymer, which has the advantages of low cost, environmentally friendly, high activity utilization. This study will provide theoretical basis and application guidance not only for expanding the application of traditional agricultural products (tea), but also for the use of natural abundant fibrous source. Moreover, it will have great significance on ensuring food safety and protect national health.