通过支架材料中功能组分对免疫细胞活性的“主动”调控，促进体细胞（种子）增殖和胞外基质合成，达到快速修复缺损的目标，是组织工程和再生医学未来的研究热点之一。在前期探明海藻多糖-昆布多糖抗感染活性的基础上，本项目利用它具有刺激免疫细胞产生特定细胞因子的特性，以成纤维细胞为体细胞模型，通过免疫细胞/体细胞共培养、细胞分子生物学等手段弄清昆布多糖调控免疫细胞释放特定因子（如IL6，IL-10等），促进成纤维细胞的快速增殖和胞外基质合成机制和构效关系，并设计和电纺出含昆布多糖免疫活性支架。以修复糖尿病大鼠皮肤溃疡组织为模型，检验所设计功能支架对慢性难愈皮肤缺损的修复能力，为最终获得一类具有免疫调控活性支架奠定基础。该研究弄清昆布多糖“主动”调控免疫细胞活性的构效关系，不仅具有重大科学意义,还为寻找新型功能再生支架开辟新途径，同时也为困扰临床上缺损组织快速修复问题的解决提供新技术，预期有广阔应用前景。

The scaffold with functional component of active immunomodulatory on immune cells, and for designing tissue regeneration scaffolds with immunomodulatory functions，which promotes proliferation and differentiation of adult cells and synthsis of extracelluar matrix, are one of the hot spots of biomaterials. In this project, laminarin, a kind of seaweed polysaccharide with anti-infection activity as we had disclosed in a former NNSFC, is designed to stimulate immunocytes to produce specific cytokines (such as IL6,IL-10) for promoting proliferation and ECM synthesis of fibroblasts with fibroblasts as model cells, and co-culture of raw264.7/fibroblasts, and other celluar and molecular biology methods. After the relationship of immunomodulation of laminarin polysaccharide and its derivatives with different molecular structures with the expressions of cytokines and growth factors, will be evaluated and disclosed with RAW264.7, mouse peritoneal macrophage,208F mouse fibroblasts, the fibrous scaffold with collagen I will be designed by co-electronspunning laminarin or its modifications and collagen I with adjusting components and electronspun time. Also, the favorable co-electronspunning membrane was chosen by testing the fibrous membrane stimulating immune cells expressing cytokines which upregulates adult cells expressing ECM and tissue repair. Skins of normal and diabetic mice II as organ models, are designed to test the immunomodulated membrane to accelerate the skin regeneration. This study is expected to clarify the structure-activity relationship between immunomodulation of materials and tissue repair acceleration, find a new way to the tissue regeneration scaffold research, and provide new materials and new methods for the rapid regeneration of skin tissue.