马立克氏病毒感鸡胸腺转录组学及其相关新功能基因的发现

马立克氏病毒(MDV)是一种细胞结合型肿瘤性疱疹病毒，引起鸡的T淋巴增生性疾病，有关致病机制仍不清楚。基因芯片的高通量能够呈现不同刺激的基因表达谱，获得病毒和宿主差异变化的基因。本项目通过感染MDV鸡胸腺，提取RNA并反转录，利用基因芯片技术研究病毒和宿主基因水平的调控机制，并用荧光定量PCR和病毒基因缺失等技术方法验证，发现新的功能基因，提出病毒与宿主相互作用，肿瘤发生发展的网络机制，为MDV 控制提出新的理论基础，研究结果对比较医学也具有重要意义。

Marek's disease virus (MDV) is a cell-associated,oncologic hepersvirus which causes a T lymphocytes proliferation in chickens. However the pathogenesis of the virus is still not very clear.The high throughput of microarray could provide a gene expression profile to differen stimulation. With this new technology we could find new genes during the interreactions between virus and host.In this project we will infect chickens with MDV RB1B and then isolate the RNA from thymus and make it reverse transcript. We will investigate the different gene exprssion and gene regulation during the chicken infected with very virluent MDV by transcripional profile analysis. The results will be confirmed by real time PCR. The function genes will be confirmed by mutant virus. Our results will provide new theory in MDV control and also will be very important in comparative Medicine