牡丹是我国的传统名花，自然花期短且集中限制了产业发展，因此，培育多季开花牡丹资源具有重要意义。秋发牡丹花芽分化早、易于二次成花。实践证明，夏秋季相对低温可以加速花芽分化进程。FT与SVP基因为温度成花途径中可互作的重要调控因子，申请人已获得部分已知功能的PsFT与PsSVP基因，但秋发牡丹中可能还存在其它同源基因，其互作的同源基因种类与模式等尚不得知。本项目以‘秋发1号’为研究材料，以非秋发品种‘洛阳红’为对照，依托已有的转录组数据库，继续挖掘FT和SVP同源基因，通过拟南芥验证其生物学功能。通过不同温度处理调控花芽分化进程，同时采用酵母单杂交、VIGS等技术确定SVP与FT的互作效应；最后综合形态解剖学、内源生理物质变化、RNA原位杂交及FT下游基因的动态表达，解析秋发牡丹SVP-FT互作响应温度信号，进而调控花芽分化的分子机制。本研究将为精准花期调控，培育多季开花牡丹资源提供理论依据。

Tree peony is an important traditional ornamental plant in China. However, Industrial production is greatly limited due to its relative short and concentrated flowering period. Therefore, it is of great value to cultivate new tree peony cultivar with multi-season flowering character. Autumn-flowering tree peony has the characteristics of early bud differentiation and easy reblooming in autumn. Practices have proved that relative low temperature in late summer and autumn would accelerate the process of flower bud differentiation. FT and SVP are important regulator genes with mutual interaction in temperature-regulated flowering pathway. We have studied some PsFT and PsSVP homologous genes with known functions, but still do not know if there are other homologous genes, neither the putative interacting genes with them. In this study, the tree peony ‘Qiu Fa No. 1’ will be used as the research material, and the non-autumn-flowering cultivar ‘Luo Yang Hong’ as the control. According to our previous transcriptome database, we will further excavate new FT and SVP homologous genes, and confirm their biological functions by Arabidopsis transgene system. Then, we plan to use various temperature treatments to regulate bud differentiation process, and confirm the interaction mode between SVP and FT by yeast one-hybrid technique, VIGS and so on. Finally, we tend to understand the molecular mechanism of the interaction of SVP and FT of autumn-flowering tree peony in responding to temperature signaling by in situ hybridization，physiology changes and spatial expression of downstream genes of FT. The study may provide more theoretical basis for precise regulation of flowering time and cultivation of multi-season flowering of tree peony germplasm resource.