不同类型淡水水体原生动物生物多样性比较研究

微生物对维持生态系统中的物质循环、能量流动及信息传递等具有重要作用。本申请项目拟以长江中游水生态系统中主要的几种淡水水体，即河流、湖泊和池塘为研究对象，拟从物种多样性和遗传多样性两个层面对目的水体真核微生物--原生动物生物多样性开展比较研究。首先，采用经典形态学分类鉴定方法与现代高通量测序技术相结合，利用QIIME、R语言、PAST等分析其生物多样性特征，确定不同水体中原生动物广布种以及特有种；通过检测原生动物群落系统发育信号，进而结合各项环境水体理化指标和相关的生物因子进行PCA整合分析，解析控制不同水体中原生动物生物多样性及其群落时空变化的主要因素。因此，本项研究不仅有望阐明不同水体中原生动物群落形成的生态学过程及机制，还将形成长江中游不同淡水水体原生动物图鉴，为水环境生态评估和保护提供参考资料。

Microbial organisms play key roles in maintaining multiple ecosystem functions of the biogeochemical cycling. In this proposed project, by using the high-throughput sequencing technologies, as well as traditional taxonomic methods, we aim to sample from three different types of freshwater bodies (i.e., rivers, lakes and ponds) in the middle reach of Yangtze River (1) to compare the species and genetic diversity of protozoa among different habitats; (2) to determine the widespread and endemic species in different freshwater bodies (using QIMME, R and PAST); (3) to uncover the environmental factors by principal component analysis (PCA) in combination with testing the phylogenetic signals in R, which might be responsible for the shaping of protozoa distribution patterns. This study will not only enhance our understanding of microbial eukaryotic diversity and distribution patterns in freshwater environments, but will also contribute to decipher the potential function and mechanism within their ecosystem. In addition, new data of this study will establish a base to apply this knowledge for the sustainability, maintenance and efficient utilization of the aquatic ecosystems.