基于微流控纸芯片分子印迹技术检测环境藻类毒素研究

随着工业的发展，环境污染也日趋严重，其中以藻类毒素为代表的环境污染的大型事件不断增多，对其的监测和检测已经成为国家的重要研究课题，本项目针对环境藻类毒素，结合微流控纸芯片技术的试剂消耗量小、通量高、便携化、易于现场检测等优势，利用分子印迹的高选择性、高特异性检测的特点，制备适合的分子印迹材料，通过化学键合和多层包裹方法构建适宜的、具有特异识别能力的分子印迹荧光量子点壳层结构，研究其分子识别过程和信号检测机制，探索用于藻类毒素检测的分子印迹新技术与新原理，最终充分有效发挥两种技术的特长，发展基于微流控芯片的环境污染物检测的新型藻类毒素快速检测技术，具有非常广阔的应用前景；该研究对提高化学事故应急救援与处置能力也具有重要的现实意义。

With the industrial development, environmental pollution has become increasingly serious, the monitoring of algae toxin pollution has become an important topic in the country. This project focus on the specific environmental pollutant algae toxins, and combined with the paper-based microfluidic's advantages such as low reagent consumption, high throughput, portability and on-site detection, the use of highly selective molecularly imprinted advantages of high specificity detection, preparation for molecularly imprinted material by chemical bonding and construction of appropriate wrapped multi-layer, and make the molecularly imprinted shell structure with specific recognition fluorescence quantum dot, to study the molecular recognition processes and signal detection mechanism, and explore new molecular imprinting technology and new principles for the detection of algal toxins. Based on the development of new environmental pollutants algae toxin on microfluidic platform,it has a very broad application prospects. The study also has important practical significance for improving chemical accident emergency rescue and processing capacity.