对虾非编码小RNA（miRNAs)在抵抗病毒侵染方面起重要作用。 而病毒(WSSV)在侵染对虾后，产生病毒自身的miRNAs。病毒miRNAs功能是什么，病毒侵染宿主过程中，是否可以利用病毒miRNAs调控宿主基因，达到免疫逃逸，是病毒和宿主互作调控有待进一步探索的重要内容。 本课题拟研究病毒miRNAs(miRNA-N6, miRNA-N13, miRNA-N23)调控对虾Toll通路基因。 研究内容包括：病毒感染后miRNA表达量检测、miRNA过表达或抑制后病毒感染的检测、miRNA作用的信号通路（miRNA与靶基因互作，包括在细胞内研究miRNA与靶基因的直接互作以及miRNA过表达或抑制表达后在对虾体内检测靶基因的表达）。通过该研究，不仅可以揭示病毒利用miRNAs进行免疫逃逸的机制，而且有利于利用病毒miRNAs作为防治靶标，抑制其表达，提高对虾抗病力，促进对虾养殖业健康发展。

Small non coding RNA (miRNAs) from shrimp play an important role in innate immune defense against virus infection. DNA virus such as white spot syndrome virus (WSSV) could produce virus-originated miRNAs during the virus infection processes. The function of virus miRNAs and whether virus can use virus-originated miRNAs regulating host immune-related genes to escape host immune attack are needed to be further explored. The projects focus on virus miRNAs (miRNA-N6, miRNA-N13, miRNA-N23) regulating host genes from Toll signal pathway. The research contents include: miRNA detection after virus infection using northern blot, detection of virus infection post over-expression or inhibition of virus miRNAs, miRNAs targeted signal pathway (miRNA and target gene interaction, including the direct interaction of miRNAs and their target genes in insect High Five cell lines and detection of expression of target genes post over-expression or inhibition of virus miRNAs in shrimp). Through this study, it can not only reveal the mechanism of virus immune escape by using virus miRNAs, but also conducive to the use of virus miRNAs as the control target, inhibition of its expression to improve the disease resistance of shrimp, promote the healthy development of shrimp aquaculture.