比率型电化学传感器因其能够利用两电流峰的比值进行定量，测定结果不随探针浓度、电极状态等环境因素的变化而变化，具有更高的稳定性和准确率，在生化传感领域具有广阔的应用前景。本项目拟基于申请人前期的研究经验，利用螺吡喃分子易发生互变异构的特性，设计并合成新型的基于一体化螺吡喃衍生物探针的比率型电化学生物传感平台。从中性分子、阴离子和阳离子目标物中各选择一种作为代表，通过引入相应的识别位点，以二茂铁作为内标型电活性基团，合成对应于三种目标物的双电流信号螺吡喃衍生物探针。其次，通过碳纳米材料的桥连作用与增强电子传输作用构建复合物修饰的玻碳电极，建立复杂体系中目标物的比率型电化学检测方法。最后，对于方法的检测灵敏度、选择性与稳定性等性能进行评价。本项目的完成将提出螺吡喃衍生物探针设计的新策略和新方法，促进电化学有机分子探针的发展，为新型电化学生物传感平台的研究提供思路。

The quantitative detection results of ratiometric electrochemical sensor were unchangeable with the concentration of probe and the electrode status, because they were based on the ratio values of two peak current intensities. Thus it has a higher stability and accuracy, and was broadly applied in the field of biochemical sensing. In this project, we proposed to construct a ratiometric electrochemical .detection platform utilizing the isomerism character of spiropyran, which is our ongoing interest. Three targets which were selected form neutral molecules, anions and cations rspectively were researched as model targets. Ferrocene was introduced as an internal standard redox group, three kinds of dual-electrical signal spiropyran probe were synthesized by designing recognition site to the corresponding targets. Then, the cross linking and enhancing electron transferring effects of carbon nanomaterials were used to construct a spiropyran derivatives modified glass carbon electrode, the ratiometric electrochemical detection method in complex matrix was established. Finally, the sensitivity, selectivity and stability of the proposed method were evaluated. The achievement of this project will put forward a new strategy for the design of spiropyran derivatives, promote the development of electrochemical organic molecular probe and provide evidence for the research of novel electrochemical biosensors.