湿邪属中医六淫之一，其性粘滞、重浊。湿性粘滞主要指病情反复缠绵难愈；湿性重浊是指感受湿邪可出现头身困重，排泄物、分泌物秽浊不清等症状。本课题组在以往多个项目资助下，发现湿邪可以下调机体免疫识别与免疫应答等,并可导致肠道菌群失调，其可能是湿性粘滞的病理机制；但对“湿性重浊”还未深入研究。基于前期研究基础，本项目的科学问题是：湿邪所导致的“湿性重浊”所出现的“浊”（排泄物、分泌物秽浊不清）与肠道微环境的具体定性定量关系如何？湿邪致 “重”（头身困重等），其机体的神经内分泌、主要神经信号变化如何？“重 ”与“浊”之间的病理关联是否由于“脑-肠互动”所致？两者之间的内在关联机制如何？为了阐明上述问题，我们拟采用宏基因测序检测不同饲养环境下小鼠的肠道微环境，并检测神经递质、行为学等相关指标的变化，以期探求湿邪是如何通过改变肠道微环境来影响感官、情绪和神经信号系统的，并最终揭示“湿性重浊”的病生机制。

Dampness, with characteristic of “Sticky-stagnant” and “Heavy-turbid”, is one of the six pathogenic factors of TCM. “Sticky-stagnant” means long duration or repetition of diseases. “Heavy-turbid” can lead to symptoms such as heaviness and lassitude of the head and body, or turbid excreta and secreta and so on. With the support of previous national funds, our group found that dampness can cause down regulation of immune recognition and immune response, and also cause imbalance of intestinal flora, which is probably the pathological mechanism of “Sticky-stagnant”. However, our group has not done further research on "Heavy-turbid ". Based on the previous research, this project puts forward the following scientific problems: What is the correlation between the “Turbid” (turbid excreta and secreta) caused by dampness and the qualitative and quantitative analysis of intestinal microenvironment? When dampness causes "Heavy" (heaviness and lassitude of the head and body), what are the changes of the body’s neuroendocrine and main neural signal? Whether the pathological correlation between "Heavy" and "Turbid" is due to "brain-gut interaction"? And what is the intrinsic mechanism between "Heavy" and "Turbid"? To clarify the scientific problems above, the approach taken in this project is to use metagenomic sequencing to analyze the intestinal microflora of mice model raised in different environments and to detect the changes of related indices such as major neurotransmitters, behavioral science and so on. By applying the methods above, this project aims to explore how the dampness to affect sense, emotion and endocrine system through changing Intestinal microenvironment, and finally reveals the pathophysiological mechanism of “dampness being heavy and turbid”.