乳铁蛋白是一种重要的功能蛋白，具有多种生理活性。然而，乳铁蛋白的加工稳定性差，且消化吸收时易被胃和小肠中存在的蛋白酶分解而失活。为提高乳铁蛋白的稳定性及生物利用度，本项目采用静电纺丝技术构建负载乳铁蛋白和益生因子的结肠靶向纳米纤维膜，探讨静电纺丝条件对乳铁蛋白分子结构及生物活性的影响规律；通过构建数学模型研究乳铁蛋白的释放动力学，阐明乳铁蛋白结肠靶向释放的机理；对纳米纤维膜进行体外益生实验和体内动物实验，采用宏基因组学及荧光定量PCR等技术研究益生因子对结肠微生物菌群结构的影响规律，结合乳铁蛋白在体内吸收率的变化，阐明益生因子调控结肠微生物菌群结构变化与其影响乳铁蛋白吸收之间的机制。本研究将为乳铁蛋白结肠靶向运输及利用奠定理论基础和提供相关的科学方法，并对构建其他类似功能蛋白的静电纺结肠靶向控释体系具有借鉴作用。

Lactoferrin is a kind of important functional protein, and has many biological activities. However, its processing stabilityn is poor, in addition, it is easy to be decomposed by proteases presented in stomach and intestine during absorption which leads to its inactivation. To improve lactoferrin’s stability and bioavailability, the colon-specific nanofilm containing lactoferrin and prebiotics will be prepared by electrospinning in this study. The influence rule of electrospinning conditions on the lactoferrin molecular structure and its bioactivity will be explored. The release kinetics of lactoferrin will be investigated by establishing corresponding methmatic model, by which, the colon-specific release mechanism of lactoferrin will be clarified. The in vitro and in vivo experiments will be carried out, and the influence rule of the prebiotics on the structure of colon bacteria will be studied by the methods of megagenomics and real-time PCR. Combined the variation of lactoferrin absorption rate, the mechanism regarding the control of the structure of colon batearia by prebiotics and its influence on lactoferrin absorption will be elucidated. This study will provide the theoretical base and scientific methods for the colon-targeted delivery and utility of lactoferrin, in addition, it will also offer the reference in developing electrospun colon-specific controlled release system of other similar functional proteins.