膀胱癌易发生淋巴转移是其预后差的主要原因。我们前期发表研究证实，长链非编码RNA(lncRNAs)在其中起重要作用，调控膀胱癌侵袭转移。近期预实验结果提示：lncRNA-BAT1在膀胱癌肿瘤细胞分泌的外泌体中高表达，通过与靶蛋白hnRNP L结合，参与肿瘤微环境的免疫调控。据此，我们提出lncRNA-BAT1调控膀胱癌转移的新机制: lncRNA-BAT1通过与hnRNP L结合，在其介导的包装机制下特异性包裹入外泌体，分泌至胞外肿瘤微环境，通过内吞作用被CD8+ T淋巴细胞摄取，进而抑制CXCR3表达，诱导肿瘤细胞发生免疫逃逸，促进膀胱癌侵袭转移。本课题拟进一步采用免疫共沉淀、高通量测序和体内免疫模型构建等技术，获得外泌体介导lncRNA-BAT1通过hnRNP L诱导肿瘤细胞免疫逃逸，促进膀胱癌侵袭转移的可靠证据，为确立其作为膀胱癌分子标记和治疗新靶点提供原创性的科学依据。

The lymph node metastasis is the main reason for poor prognosis in bladder cancer. Our previous studies confirmed that long non-coding RNAs (lncRNAs) play an important role in bladder cancer metastasis. Recently, our results demonstrated that lncRNA-BAT1 is highly expressed in cancer-derived exosomes and involved in the immunoregulation of tumor microenvironment by binding to hnRNP L in bladder cancer cells. Herein, we propose a novel mechanism of lncRNA-BAT1 in promoting bladder cancer metastasis. LncRNA-BAT1 is specifically encapsulated into exosomes and secreted to extracellular tumor microenvironment by interacting with hnRNP L. Then it is uptaken by macrophage endocytosis, enhancing the expression of CCR5 and tumor immune escape, and therefore promotes bladder cancer metastasis. In the present study, through RNA pull down, high through-put sequencing and in vivo model, we will further confirm that lncRNA-BAT1 is involved in tumor immune escape via hnRNP L and promotes the bladder cancer metastasis. The success of this study may reveal new mechanisms for bladder cancer metastasis in the future.