植物核纤层类似蛋白在DNA损伤修复中的功能及其分子机制

为维持基因组的完整性与稳定性，生物体启动DNA损伤反应(DDR)，进而进行DNA的损伤修复。核纤层作为细胞核骨架，锚定染色质与蛋白复合体，参与调控细胞核中包括基因复制、转录、修复等的众多生物学过程。植物中存在核纤层类似蛋白(CROWDED NUCLEI, CRWN)，对其功能和作用机制却知之甚少。我们前期在发现CRWN蛋白参与植物抗病基础上，进一步发现crwn1 crwn2双突变体具有DNA 损伤相关的表型。分子水平上CRWN1与染色体结构维持复合体(SMC) 5/6中的组分SNI1存在蛋白上的直接互作；遗传关系上crwn1 crwn2 sni1三突变体发育异常、植株严重矮小。我们拟在长期研究植物细胞核结构和功能工作基础上，探明CRWN如何调控DNA损伤修复，同时揭示CRWN在抗病与DNA损伤修复交叉通路中的功能。

To maintain genome integrity and stability, the DNA damage responses (DDR) are triggered to carry out DNA damage repair. The nuclear lamina functions as a scaffold, tethering chromatin and protein complexes, and regulating a variety of nuclear processes such as gene replication, transcription and DNA repair. Plants encode lamin-like proteins, CROWDED NUCLEI (CRWNs) with their functions largely unknown. In addition to the role of CRWNs in plant immunity, we found that crwn1 crwn2 double mutant show DNA damage related phenotypes. At the molecular level, CRWN1 directly interacts with SNI1, a subunit of the STRUCTURAL MAINTENANCE OF CHROMOSOMES (SMC) 5/6 complex. Genetically, crwn1 crwn2 sni1 triple mutant shows abnormal growth and severally dwarfing phenotypes. Based on our long-term studies on plant nuclear structures and functions, we plan to reveal how CRWN regulates DNA damage repair and further uncover the role of CRWN in the crosstalk between plant pathogen resistance and DNA damage and repair.