阻塞性睡眠呼吸暂停低通气综合征（OSAHS）的发病机制尚未完全明确，颏舌肌功能下降可能在OSAHS发病过程中起重要作用，而延髓的舌下神经运动核（HMN）直接控制颏舌肌的神经输出量，影响颏舌肌功能。多巴胺在睡眠-觉醒调控中发挥了重要的作用，其功能异常可导致严重的睡眠障碍。我们的前期研究发现多巴胺能使HMN产生明显的兴奋效应，并且这种兴奋作用具有剂量效应，提示多巴胺可能通过调节舌下神经运动核的兴奋性参与了OSAHS的发病机制，但具体调节机制尚不清楚。本课题拟通过免疫荧光与激光共聚焦显微镜技术定位HMN多巴胺受体，运用全细胞膜片钳技术明确多巴胺对HMN的兴奋作用及多巴胺受体的敏感性，鉴定产生兴奋作用的受体类型，同时探讨其调节机制及信号通路，并结合在体舌下神经核微透析技术进一步验证多巴胺对HMN的兴奋作用，为探讨OSAHS的发病机制及药物治疗提供理论依据。

The pathogenesis of obstructive sleep apnea hypopnea syndrome (OSAHS) is still not completely clear. The functional decline of the genioglossus muscle may play an important role in the pathogenesis of OSAHS , and hypoglossal motor nucleus (HMN) directly control the neural output of the genioglossus muscle and affect the genioglossal function. Dopamine plays an important role in sleep-wake regulation, and its dysfunction can lead to severe sleep disorders. Our previous study found that dopamine produced a significant excitatory effect on HMN, and this excitatory effect had a dose effect, suggesting that dopamine may be involved in the pathogenesis of OSAHS by modulating the excitability of the HMN, but the specific regulatory mechanism is not clear. In this study, the dopamine receptors of HMN will be localize by immunofluorescence and laser confocal microscopy. The excitatory effects of dopamine on HMN , the sensitivity of dopamine receptors and the type of excitatory receptor will be identified by whole-cell patch-clamp technique and its regulatory mechanism and signal pathway will be explore simultaneously. To further verify the excitatory effects of dopamine on HMN, the hypoglossal nucleus microdialysis technique in vivo will be taken, as to provide a theoretical basis for the pathogenesis and drug treatment of OSAHS.