牙槽骨量不足是牙种植治疗的难点，Onlay植骨是临床常用的牙槽骨增量技术，自体Onlay骨块存在来源有限、创伤大、难以精确塑形的弊端，亟需探索一种个性化人工骨来满足理想Onlay植骨的需求。本课题拟以PLGA、可降解骨替代材料DCPD和bio-MOF/DMOG载药体系为打印基质，采用3D打印制备一种新型的负载金属-有机骨架(bio-MOF)药物缓释系统的个性化Onlay人工骨块。该骨块能精确匹配缺损部位外形，能调控内部孔隙以促进营养物质运输、成骨细胞攀附及血管组织生长；bio-MOF载药体系具有优良的生物相容性、生物降解性及三级药物缓释特性，能缓释Zn2+和促血管生成药物DMOG，进一步优化人工骨块的抗菌性、成骨性及成血管性。本课题通过体外和体内动物模型，对此新型个性化人工骨块的理化特性、生物学性能、修复牙槽骨缺损的成骨机制进行探讨，为解决牙种植骨量不足临床难题提供重要的实验基础和理论指导

Alveolar bone defect is one of the most considerable problems in rehabilitation with dental implants. Onlay block grafting is currently the most reliable technique for alveolar bone augmentation. However, autologous onlay bone graft has disadvantages such as donor site morbidity, high resorption rate, and limited bone availability. Therefore, artificial bone onlay grafts are being developed to overcome the limitations of autografts. In this study, a novel customized onlay graft doping with bio-MOF based drug delivery system is to be fabricated for the restoration of alveolar bone defect via three dimensional printing technique. This customized onlay graft can accurately match the shape of defect site as well as facilitate the transportation of nutrients and the growth of both bone and vascular tissues through regulating the internal pore size and porosity. Additionally, bio-MOF has excellent biocompatibility, biodegradability and controlled drug-release effect, which can slowly release the zinc ions and angiogenic drugs, improving the osteogenesis, angiogenesis and antibacterial properties of the artificial onlay graft. In this study, the physicochemical properties, biological performance, and osteogenic mechanism of the customized onlay graft will be investigated systematically in vitro and in vivo, providing an important experimental basis for the restoration of alveolar bone defect.