多色荧光/光声聚合物纳米探针的构建及其肺癌淋巴转移精准诊断研究

手术治疗是早期肺癌的首选治疗方式，对肺癌淋巴转移的精准诊断是确定患者治疗方案以及影响预后的关键，然而目前临床影像学方法尚难以对转移淋巴结实现精准诊断，因此，亟需发展高特异性、高灵敏度及高分辨率的分子影像学方法及分子探针以实现对肺癌淋巴结转移程度的准确评估。申请人前期研究设计合成了一类近红外荧光(NIRF)聚合物纳米探针可在体检测裸鼠12个淋巴结。本项目拟在已有工作基础上，以红色MEH-PPV、黄色PFBT、绿色PFPV聚合物为载体，以肺癌细胞过量表达的受体为靶点，构建双/多受体特异性靶向的多色荧光聚合物纳米探针。红/黄/绿多色荧光用于多通道荧光显微成像及流式定量分析。通过掺杂NIR染料获得NIRF/光声双模态聚合物纳米探针用于活体成像，结合NIRF成像技术的高灵敏度以及光声成像技术的高空间分辨率，实现对肺癌淋巴转移的精准诊断。本项目的结果将为肺癌淋巴转移影像学早期诊断提供新方法。

Surgery is the primary treatment for early stage lung cancer. Correct evaluation of the presence or absence of metastases in intrathoracic lymph nodes is a critical factor that determines the treatment options and selects patients who are likely to benefit from surgery in patients with lung cancer. However, to date, it is difficult to realize the precise diagnosis of metastasis lymph nodes by using the current clinical imaging technologies. Therefore, there is an urgent need to realize the precise diagnosis of metastasis lymph nodes of lung cancer by development of targeted probe with high specificity, high sensitivity and high resolution. Previously, we have developed near-infrared fluorescent (NIRF) polymer nanoprobe for imaging twelve lymph nodes of a mouse at one time. In this project, we purpose to develop receptor-targeted nanoprobes based on red MEH-PPV, yellow PFBT and green PFPV polymers by choosing overexpressing receptor of lung cancer as targets. Red/yellow/green multicolor fluorescence can be used for multi-channel fluorescence microscope imaging and flow cytometry analysis. By doping NIR dye to get NIRF and photoacoustic nanoprobes. And the precise diagnosis of metastasis lymph nodes of lung cancer can be realized by combine the NIRF imaging of high sensitivity and photoacoustic imaging of high resolution. The result of this study will provide a unique approach for the early diagnosis of lymph node metastasis in lung cancer in vivo.