转录因子BoMYB28对青花菜短链脂肪族硫苷生物合成起重要的调控作用，但其参与亚硒酸钠调控萝卜硫素积累的分子机制尚不明确。本项目拟对青花菜进行外源亚硒酸钠处理，对比分析BoMYB28基因和萝卜硫素合成关键基因（BCAT4、MAM1、MAM3、CYP79F1、CYP83A1、MY等）的时空表达特性，筛选转录因子BoMYB28的候选靶基因，并采用酵母单杂交、EMSA和ChIP-qPCR验证转录因子与候选靶基因启动子在蛋白质-DNA水平上的互作关系。基于此，对BoMYB28基因的过表达和抑制表达青花菜植株进行外源亚硒酸钠处理，分析BoMYB28和靶基因的时空表达特征以及萝卜硫素合成过程中关键代谢物的变化规律。通过转基因验证，解析转录因子BoMYB28参与亚硒酸钠调控青花菜萝卜硫素积累的分子机制，进一步丰富青花菜品质发育和形成机理，为品质调控和优质栽培提供科学的理论依据。

Transcription factor BoMYB28 plays an important role on regulating biosynthesis of short-chain aliphatic glucosinolate in broccoli. But molecular mechanism remains unclear that how BoMYB28 involved in sodium selenite regulation of broccoli sulforaphane accumulation. To screen candidate target genes of transcription factor BoMYB28, the spatio-temporal expression characteristics between BoMYB28 and key genes of sulforaphane synthesis are analyzed in broccoli under sodium selenite treatment. Then, yeast one-hybird assays, EMSA and ChIP-qPCR are used to study the protein-DNA interaction of BoMYB28 with promoters of candidate target genes involed in sulforaphane biosynthesis. Based on this, the transgene broccoli of overexpression and inhibition of BoMYB28 gene were treated with sodium selenite. The spatio-temporal expression characteristics of BoMYB28 and target genes and the changes of key metabolites in the process of sulforaphane synthesis are analyzed to reveale the molecular mechanism of BoMYB28 involved in sodium selenite regulation of broccoli sulforaphane accumulation. The results obtained by this project can enrich the development and formation mechanism of broccoli quality, further provide scientific theory basis for high quality regulation and cultivation.