J亚群禽白血病是由ALV-J引起的一种免疫抑制性肿瘤性疾病，严重危害养禽业。本团队首次发现在感染ALV-J的公鸡精液外泌体中存在ALV-J p19基因编码的miRNA（命名为ALV-miRNA-p19-01），研究表明该miRNA具有促进ALV-J出芽的作用，且DUSP6是其靶基因。研究报道DUSP6可通过抑制ERK酶活性调控ERK信号通路，而ERK信号通路与多种病毒的出芽和释放密切相关。基于这些研究基础，本项目将深入研究ALV-miRNA-p19-01促进 ALV-J出芽的调控机制，探讨该miRNA靶向DUSP6对ALV-J出芽的影响，以及该miRNA靶向DUSP6调控ERK信号通路对ALV-J出芽的影响，阐明ALV-miRNA-p19-01调控DUSP6/ERK信号通路促进ALV-J出芽的分子机制。本项目研究成果对J亚群禽白血病的防控有重要的理论和实践意义。

Avian leukosis subgroup J is an immunosuppressive oncogenic infectious disease caused by avian leukosis virus subgroup J (ALV-J), which is responsible for serious harm to poultry industry. Our team firstly found that exosomes derived from seminal plasma of rooster infected with ALV-J contain a microRNA encoded by ALV-J p19 gene and named it as ALV-miRNA-p19-01. Our previous study has demonstrated that this miRNA can promote the budding of ALV-J and its target gene is DUSP6. Previous studies have demonstrated DUSP6 can inhibit the activity of ERK, whereby regulating the function of ERK signaling pathway, which is closely related to the budding and release of a variety of viruses. Based on these research results, the aim of our project is to further study the regulatory mechanism of ALV-miRNA-p19-01 promoting ALV-J budding. We will investigate the effect of this miRNA targeting DUSP6 on ALV-J budding, and further investigate the effect of this miRNA targeted DUSP6 regulating ERK signaling pathway on ALV-J budding, and finally elucidate the molecular mechanism of ALV-miRNA-p19-01 promoting ALV-J budding through regulating DUSP6/ERK signaling pathway. The research results of this project will definitely have an important theoretical and practical significance for the prevention and control of avian leukosis subgroup J.