乳蛋白是衡量牛奶营养品质的重要指标，缓解乳腺炎性损伤对于实现奶牛健康泌乳具有重要的应用价值。乳源活性肽VPP和IPP兼具营养和减缓炎症反应的功能，然而其在奶牛乳腺损伤修复和乳蛋白合成中的效果评价，以及靶向受体研究却刚刚起步。本项目在验证乳源抗炎肽可缓解奶牛乳腺损伤和修复乳蛋白合成的基础上，利用奶牛乳腺上皮细胞模型，通过链霉亲和素标记磁珠的免疫共沉淀结合质谱技术，获得抗炎肽的特异性受体并探讨其功能特性，解析受体调节乳蛋白合成的作用机理。通过奶牛生产试验和活体取样技术，进一步验证抗炎肽及其受体对奶牛乳腺损伤修复和乳蛋白合成的调节作用，为奶牛乳腺健康高效泌乳和改善乳品质提供理论依据，并为反刍动物活性肽添加剂的开发应用提供参考。

Milk protein is one of the major nutritional components associated with milk quality. It is of great practical value to alleviate inflammatory injury for healthy lactation in dairy cow. Milk-derived peptides VPP and IPP not only serve as precursors for protein synthesis, but also act as anti-inflammation agents. However, the effects of these two milk-derived peptides on inflammatory injury and milk protein synthesis, as well as their specific receptor have not been studied in bovine mammary epithelial cells. In this proposed study, based on the anti-inflammatory activity and enhancement of milk protein synthesis of milk-derived peptides, the co-immunoprecipitation combined with mass spectrometry approach will be employed to analyze the specific receptor and its function. The regulatory mechanisms of receptor on milk protein synthesis will be revealed in bovine mammary epithelial cells. The positive effects of milk-derived peptides and their receptor on anti-inflammation and milk protein synthesis will be further confirmed in dairy cows. The aim of this proposal is to provide deep insight for improving milk quality and the application of bioactive peptides in dairy cow.