课题组研究发现防龋抗龋药物氟化氨银虽然可以促进牙本质龋的矿化修复，但存在牙本质胶原纤维矿化不全、矿化机理不明等问题。据此本项目提出“银氨离子与氟离子共存具有协同作用。一方面，参与了调控无机矿物羟基磷灰石晶体的成核生长取向；另一方面，通过相互作用改变了胶原纤维分子的结构，使之与无机矿物晶体成核生长相匹配，从而促进矿化”的科学假说研究其矿化机理。研究围绕牙本质矿化的两个关键因素（羟基磷灰石晶体的生长、胶原纤维矿化模板作用）展开。首先，采用化学动力学方法研究氟化氨银在磷酸钙盐沉积过程中的调控作用；其次，评价氟化氨银对胶原蛋白分子自组装的结构调控作用、采用化学动力学方法研究氟化胺银对自组装的胶原纤维矿化的调控作用。最后，在获得矿化机理参数的基础上，对氟化氨银进行纳米微粒化改性，进一步考察其对胶原纤维的修复矿化作用，防龋抗龋作用。本研究阐明氟化氨银的抗龋机理，将为进一步临床应用技术改进奠定基础。

Silver diamine fluoride (SDF), unlike other fluoride products which prevent the formation of new caries, is also capable of halting the caries process efficiently. However, the mechanism behind this success is unclear and it stains the caries lesion. Understanding the process of mineral deposition and formation is imperative to the development of treatments for caries, may also have important implications for the design of bioinspired materials. This study aimed to investigate the role of SDF in this mineralisation process and improve the technology. This study will investigate the mechanism of SDF on mineral crystal formation, the mechanism of SDF on collagen mineralisation, remineralisation effect of SDF on demineralised dentine collagen matrix. Different concentrations of SDF will incorporated into different phases of mineral crystal formation process, assembled cross-linked collagen, or human dentine matrix, silver nitrate and potassium fluoride will be used as controls. By understanding the mechanisms of SDF, we will modify SDF molecular using nanotechnology, the modified SDF is expected to have a favorable color effect and improved mineralisation effect on dentine caries. Multiple advanced technologies like cryo-transmission electron microscopy, scanning electron microscopy, dynamic light scattering, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, atomic force microcopy will be used to characterize the mineral crystal, collagen as well as the remineralisation process.