枸杞是我国珍贵的药食两用物品，其主要成分枸杞多糖（LBP）已被证实具有降糖等生物学作用。多糖类物质仅有小部分以大分子形式吸收，主要是经过降解后以小分子的形式进入体内的。LBP作为一种蛋白多糖，吸收率是多少，是如何被吸收，代谢生成何种物质发挥降血糖作用的，目前尚不清楚。明确LBP等多糖类物质的吸收与代谢的过程，是进一步明确LBP等多糖功效发挥途径的关键。.本项目拟采用荧光标记法对LBP进行标记，给予SD大鼠进行LBP的代谢动力学研究，同时采用代谢组学方法研究给予LBP后大鼠体内代谢谱的变化，并给予20-40岁正常健康男性口服非标记的LBP，研究对人体代谢谱的影响，与动物试验的结果进行比较，分析LBP代谢分解后的主要组分，并利用LBP及其主要代谢组分进行体外细胞研究，探讨LBP发挥降糖功效的机制。本研究成果将对多糖类物质功效作用的机制研究提供新的思路，为深入研究和利用多糖类物质提供科学依据。

Lycium barbarum is our precious Chinese herbal medicine and the civil common food compounding. The main component of Lycium barbarum polysaccharide (LBP) has been proved to be the potential hypoglycemic biological activity. Although polysaccharides can partly be absorbed as macromolecules form in intestine, mainly be degradated into small molecules that enter the body. LBP is as a proteoglycan, we need to answer how many is absorbed in intestine, how to enter the body, what metabolites be formed in the body which have the hypoglycemic activity. And these are currently unknown. Therefore, to study and understand LBP or other polysaccharides absorption and metabolism processes is the key to further clarify the active effects of the LBP and the polysaccharides...In this project LBP will be marked with fluorescence labeling and the labeled LBP will be given to the SD rats for studying its metabolic kinetics. At same time, we will use the metabolomics method to study the change of the SD rats' metabolic profile after the administration of the LBP. Then 20-40 years old normal healthy male will be given non-labeled LBP to study the human metabolic profiling changes. To compare the results of animal experiment and human test, the main metabolites of LBP will be identified. Finally, the LBP and its metabolites will be used to to study the mechanism of LBP hypoglycemic efficacy in vitro with different cell model. The results of this research will provide new ideas for the mechanism research of the polysaccharides biological activity, and provide the scientific basis for further research and utilization of polysaccharides.