二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)和ɑ-亚麻酸（ALA）分别为动植物来源的ω-3多饱和脂肪酸（PUFA），EPA和DHA对改善脂代谢、抗炎等作用研究较多，但ALA的重要性被忽视。2型糖尿病（T2DM）常伴有脂代谢紊乱，有关两种来源ω-3PUFA对T2DM合并脂代谢异常糖脂代谢及Toll样受体（TLR）信号通路影响的比较研究尚未见报道。本项目拟对T2DM合并脂代谢异常人群进行ALA和鱼油（DHA+EPA）干预，并以自发性KKAy自发性T2DM模型小鼠和MyD88基因敲除小鼠研究不同来源ω-3PUFA对糖脂代谢、脂肪细胞因子水平及TLR4/NF-κB通路MyD88依赖型和/或MyD88非依赖型途径相关成分的影响，探讨不同来源ω-3 PUFA对糖尿病糖脂代谢的影响及其作用途径。研究成果将为阐明不同来源ω-3 PUFA对炎症信号通路的调节途径进而引起糖尿病发生发展的机制提供理论依据。

Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and ɑ-linolenic acid (ALA) are omega-3 polyunsaturated fatty acids (ω-3PUFA) respectively from animal and plant sources. There are many studies about effects of EPA and DHA on improving lipid metabolism and inflammation, however, the importance of ALA is neglected. Type 2 diabetes (T2DM) is often accompanied with lipid metabolic disorder. The comparison study of effects of different sourecs of ω-3PUFA on glucose and lipid metabolism in T2DM and their signaling pathways have not yet been reported. This project is designed to give intervention using ALA and fish oil (DHA + EPA) for T2DM population accompanied with lipid metabolic disorder. Meanwhile, the spontaneous type 2 diabetic KKAy mice and MyD88 gene knockout mice are used to study the effects of different sources of ω-3PUFA on glouse and lipid metabolism, adipocytokines, related factors to TLR4/NF-kappa B pathway (MyD88 dependent and/or MyD88 independent way), so as to exploring the effects of different sources of ω-3PUFA on glucose and lipid metabolism and their mechanism for diabetes. The results of this project will help to illuminate the role of ω-3PUFA in inflammatory pathway, and furthermore to provide theoretical basis in the development of diabetes.