The prevalence of Coxiella burnetii shedding in dairy goats at the time of parturition in an endemically infected enterprise and associated milk yield losses.
The prevalence of Coxiella burnetii shedding in dairy goats at the time of parturition in an endemically infected enterprise and associated milk yield losses.
复制标题
DOI：
10.1186/s12917-018-1667-x
复制
发表时间：
2018-11-20
影响因子：
2.6
通讯作者：
Stevenson MA
中科院分区：
文献类型：
--
作者：
Canevari JT;Firestone SM;Vincent G;Campbell A;Tan T;Muleme M;Cameron AWN;Stevenson MA
这是一项针对澳大利亚维多利亚州地方性奶山羊企业伯内特氏衣原体感染流行情况的小组研究。我们的第一个目标是确定母鹿在分娩时脱落伯内特氏菌的流行率，并量化每个伯内特氏菌阳性样本中存在的基因组当量 (GE) 的浓度。我们的第二个目标是确定持续脱落的积极行为的比例。我们的最终目标是量化产奶时伯纳特梭菌 qPCR 状态与随后哺乳期间每日产奶量之间的关联。阴道拭子（n = 490）是在玩耍时收集的，并使用定量聚合酶链反应（qPCR）分析进行分析。 15%（95% CI：12% 至 18%）的样本中检测到伯氏念珠菌脱落。根据 qPCR 估计的 GE 浓度，将其分为 qPCR 阴性、qPCR 阳性低值和 qPCR 阳性高值。在随后分娩时再次取样的脱落母犬中，有 20%（95% CI：10% 至 35%）检测到浓度相对较低的持续脱落。在控制可能的混杂因素并调整个体母鹿水平的日产奶量变化后，与 qPCR 阴性母兔相比，qPCR 阳性高剂量的日产奶量降低了 17%（95% CI：3% 至 32%）（p= 0.02）。伯内特蠹的脱落浓度高度倾斜，相对较小的群体脱落的伯内特蠹的数量相对较高。此外，与 qPCR 阴性的母牛相比，高排量母牛的产奶量降低。及早发现并扑杀大量脱落的母兔将提高农场的盈利能力并降低 Q 热传播的风险。
This was a panel study of the prevalence of C. burnetii infection in does in an endemic dairy goat enterprise in Victoria, Australia. Our first objective was to determine the prevalence of does shedding C. burnetii at the time of parturition and to quantify the concentration of genome equivalents (GE) present in each C. burnetii positive sample. Our second objective was to determine the proportion of positive does that were persistent shedders. Our final objective was to quantify the association between C. burnetii qPCR status at the time of kidding and daily milk volumes produced during the subsequent lactation. Vaginal swabs (n= 490) were collected from does at the time of kidding and analysed using a quantitative polymerase chain reaction (qPCR) assay. Shedding of C. burnetii was detected in 15% (95% CI: 12% to 18%) of the sampled does. Does were classified as qPCR-negative, qPCR-positive low and qPCR-positive high based on the estimated concentration of GE from the qPCR. Persistent shedding at relatively low concentrations was detected in 20% (95% CI: 10% to35%) of shedding does sampled again at their subsequent parturition. After controlling for possible confounders and adjusting for variation in daily milk yields at the individual doe level, daily milk yields for qPCR-positive high does were reduced by 17% (95% CI: 3% to 32%) compared to qPCR-negative does (p= 0.02). Shedding concentrations of C. burnetii were highly skewed, with a relatively small group of does shedding relatively high quantities of C. burnetii. Further, high shedding does had reduced milk yields compared to qPCR-negative does. Early detection and culling of high shedding does would result in increased farm profitability and reduce the risk of Q fever transmission.
登录
查看更多内容
影响因子：
4
作者：
Capparelli, R.;Parlato, M.;Iannelli, D.
通讯作者：
Iannelli, D.
影响因子：
2.4
作者：
Astobiza, I.;Barandika, J. F.;Garcia-Perez, A. L.
通讯作者：
Garcia-Perez, A. L.
影响因子：
4.4
作者：
Barlow, John;Rauch, Brad;Schukken, Ynte
通讯作者：
Schukken, Ynte
影响因子：
2.1
作者：
Hatchette, T;Campbell, N;Marrie, TJ
通讯作者：
Marrie, TJ
影响因子：
3.3
作者：
Berri, M;Laroucau, K;Rodolakis, A
通讯作者：
Rodolakis, A