Near-infrared fluorescence imaging in the largely unexplored window of 900-1,000 nm.
Near-infrared fluorescence imaging in the largely unexplored window of 900-1,000 nm.
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在很大程度上未探索的 900-1,000 nm 窗口中的近红外荧光成像
作者：
Deng G;Li S;Sun Z;Li W;Zhou L;Zhang J;Gong P;Cai L
近红外（NIR）荧光成像依赖于在700-900 nm NIR-Ia或1，000 - 1，700 nm NIR-II窗口中发射的荧光团，用于生成深层组织图像。到目前为止，很少有为900- 1000 nm NIR-Ib窗口开发的荧光团。这主要是因为NIR-Ib光被认为被水强烈吸收。方法：在这里，我们发现六个七甲川染料在NIR-Ia和NIR-Ib窗口都有不同的发射峰。我们测试了这些造影剂的性能，通过将它们引入到常见的室内植物Epipremnum aureum的叶子中，这些植物具有来自Khaya senegalensis的早期炭疽病叶子感染，以及将它们注射到裸鼠的后足和体内荷瘤小鼠的尾部。结果如下：七甲川染料产生上级图像的叶片脉序，炭疽病感染的位置，前哨淋巴结，脑肿瘤和皮下肿瘤的NIR-Ib窗口。我们发现，NIR-Ib图像具有显著增强的信号背景比，因为生物组织和水的自发荧光、散射和光吸收在较长波长下较弱。结论：NIR-Ib荧光成像是研究植物叶片中前哨淋巴结、肿瘤、叶脉和早期炭疽病感染位置的有力方法。这些发现挑战了我们目前对近红外荧光成像的看法，并可能对生物医学研究和图像引导的癌症手术产生重要影响。
Near-infrared (NIR) fluorescence imaging has relied on fluorophores that emit in the 700-900 nm NIR-Ia or 1,000-1,700 nm NIR-II window for generating deep-tissue images. Up until now, there have been few fluorophores developed for the 900-1,000 nm NIR-Ib window. This is largely because NIR-Ib light is thought to be strongly absorbed by water. Methods: Here we found that six heptamethine dyes had distinct emission peaks in both the NIR-Ia and NIR-Ib window. We tested the performance of these contrast agents by introducing them into the leaves of the common house plant Epipremnum aureum with early stage anthracnose leaf infections from Khaya senegalensis, as well as injecting them into the hind feet of nude mice and tails of tumour-bearing mice in vivo. Results: Heptamethine dyes yielded superior images of leaf venation, anthracnose infection locations, sentinel lymph nodes, brain tumours and subcutaneous tumours in the NIR-Ib window. We found that NIR-Ib images had markedly enhanced signal-to-background ratio because autofluorescence, scattering and light absorption by biological tissues and water were weaker at longer wavelengths. Conclusion: NIR-Ib fluorescence imaging was a powerful method for studying sentinel lymph nodes, tumours, leaf veins and early anthracnose infection locations in plant leaves. The findings challenge our current view of NIR fluorescence imaging and may have important implications for biomedical research and image-guided cancer surgery.
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DOI：
10.1117/12.2011015
发表时间：
2013-03-25
期刊：
Proceedings of SPIE--the International Society for Optical Engineering
影响因子：
--
作者：
Fried WA;Fried D;Chan KH;Darling CL
通讯作者：
Darling CL
影响因子：
6
作者：
Ashitate, Yoshitomo;Tanaka, Eiichi;Stockdale, Alan;Choi, Hak Soo;Frangioni, John V.
通讯作者：
Frangioni, John V.
影响因子：
15
作者：
Harrison VS;Carney CE;MacRenaris KW;Waters EA;Meade TJ
通讯作者：
Meade TJ
影响因子：
16.6
作者：
Lei, Zuhai;Li, Xinran;Yang, Youjun
通讯作者：
Yang, Youjun
影响因子：
3.1
作者：
Hyun H;Henary M;Gao T;Narayana L;Owens EA;Lee JH;Park G;Wada H;Ashitate Y;Frangioni JV;Choi HS
通讯作者：
Choi HS