The relationship between smoking exposure and p53 overexpression in colorectal cancer.
The relationship between smoking exposure and p53 overexpression in colorectal cancer.
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DOI：
10.1038/bjc.1996.180
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发表时间：
1996-04
影响因子：
8.8
通讯作者：
Asirwatham, JE
中科院分区：
文献类型：
--
作者：
Freedman, AN;Michalek, AM;Marshall, JR;Mettlin, CJ;Petrelli, NJ;Zhang, ZF;Black, JD;Satchidanand, S;Asirwatham, JE
尽管关于吸烟与结直肠癌风险之间关系的流行病学研究一直模棱两可，但一直发现吸烟与结直肠腺瘤的发展呈正相关。我们进行了一项流行病学研究，以确定在切除时获得的肿瘤中p53蛋白过表达是否与结直肠癌中的香烟暴露有关。共有163名结直肠癌病例和326名健康对照者回答了一份关于结直肠癌风险因素的标准化问卷，其中包括他们吸烟史的详细信息。使用亲和素-生物素免疫过氧化物酶程序和多克隆抗p53抗体CM1，对所有患者的肿瘤进行了p53过表达的免疫组织化学分析。结直肠病例与对照组的比较显示，与不吸烟者相比，前吸烟者（OR = 1.34, 95% CI 0.85-2.12）和当前吸烟者（OR = 1.13, 95% CI 0.63-2.02）的风险更高。吸烟总包年未发现剂量-反应关系（趋势检验：P = 0.19）。然而，当将p53阳性病例与p53阴性病例进行比较时，发现吸烟总包年的趋势表明病因学上的异质性（趋势检验：P = 0.06）。与对照组相比，估计个体发生p53阳性肿瘤的相对风险与吸烟状况或吸烟总包年无关。然而，当p53阴性病例与对照组比较时，发现前吸烟者（OR = 1.84, 95% CI 1.00-3.37）和当前吸烟年份（趋势检验：P = 0.03）的风险升高。通过p53阳性依赖途径发展的结直肠肿瘤与吸烟暴露无关。吸烟显著增加了p53阴性独立通路的风险。P53过表达似乎与结直肠癌的吸烟暴露有关。
Although epidemiological studies of the relationship between cigarette smoking and colorectal cancer risk have been equivocal, a positive association is consistently found for colorectal adenoma development. We performed an epidemiological study to determine whether p53 protein overexpression, in tumours obtained at the time of resection, is associated with cigarette exposure in colorectal cancer. A total of 163 colorectal cancer cases and 326 healthy controls responded to a standardised questionnaire on colorectal cancer risk factors including detailed information on their history of cigarette smoking. All patients' tumours were analysed immunohistochemically for p53 overexpression using an avidin-biotin immunoperoxidase procedure and polyclonal anti-p53 antibody CM1. Comparison of colorectal cases with controls revealed an elevated risk for ex-smokers (OR = 1.34, 95% CI 0.85-2.12) and current smokers (OR = 1.13, 95% CI 0.63-2.02) when compared with non-smokers. No dose-response relationship was found for total pack-years of smoking (trend test: P = 0.19). However, a trend for total pack-years of smoking was found when p53-positive cases were compared with p53-negative cases suggesting aetiological, heterogeneity (trend test: P = 0.06). Estimating the individual relative risk of developing a p53-positive tumour relative to controls showed no associations for smoking status or total pack-years of smoking. However, when p53-negative cases were compared with controls, an elevated risk was found for ex-smokers (OR = 1.84, 95% CI 1.00-3.37) and current years of smoking (trend test: P = 0.03). Colorectal tumours developing through p53-positive dependent pathways were not associated with smoking exposure. A significant increase in risk was observed for the p53-negative independent pathway with smoking. p53 overexpression appears to be associated with smoking exposure in colorectal cancer.
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影响因子：
24.5
作者：
COPE, GF;WYATT, JI;KELLEHER, J
通讯作者：
KELLEHER, J
影响因子：
7.7
作者：
FERRARONI, M;NEGRI, E;FRANCESCHI, S
通讯作者：
FRANCESCHI, S
影响因子：
2.3
作者：
CHUTE, CG;WILLETT, WC;SPEIZER, FE
通讯作者：
SPEIZER, FE
DOI：
10.1093/jnci/86.3.192
发表时间：
1994-02-02
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
GIOVANNUCCI, E;COLDITZ, GA;SPEIZER, FE
通讯作者：
SPEIZER, FE
影响因子：
5
作者：
DALES, LG;FRIEDMAN, GD;WILLIAMS, SR
通讯作者：
WILLIAMS, SR