In situ immunohistochemical detection of intracellular Mycoplasma salivarium in the epithelial cells of oral leukoplakia.
In situ immunohistochemical detection of intracellular Mycoplasma salivarium in the epithelial cells of oral leukoplakia.
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DOI：
10.1111/jop.12215
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Nagasawa D
中科院分区：
文献类型：
--
作者：
Mizuki H;Kawamura T;Nagasawa D
支原体是最小的自由生物；唾液支原体和口支原体是从口咽部分离出来的最常见的物种。口腔白斑是口腔黏膜中最常见的潜在恶性疾病，其病因尚未确定。我们先前对DNA结合荧光染料的研究表明，在白斑组织的上皮细胞中存在支原体。我们的目的是用免疫组织化学方法检测白斑上皮细胞中的唾液分枝杆菌。我们将含有完全弗氏佐剂的唾液分枝杆菌细胞(ATCC 23064)注射到兔体内，并将含有完全弗氏佐剂的唾液分枝杆菌细胞(ATCC 23557)注射到大鼠的足垫中，制备了对支原体有反应的多克隆抗体(Pab)。然后，我们尝试用免疫组织化学方法检测白斑组织上皮中的唾液分枝杆菌。我们获得了对本研究中使用的所有七种支原体都有反应的抗支原体兔抗体。获得了3个产生抗唾液分枝杆菌特异性单抗的杂交瘤克隆，并建立了7-6H的唾液分枝杆菌特异性单抗。免疫组织化学染色显示白斑上皮细胞中有唾液分枝杆菌，组织学表现为增生和过度角化。聚合酶链式反应和测序证实白斑上皮细胞中存在唾液支原体DNA。白斑上皮细胞内可见唾液支原体。
Mycoplasmas are the smallest free-living organisms; Mycoplasma salivarium and Mycoplasma orale are the most common species isolated from the oropharynx. Oral leukoplakia is the most prevalent potentially malignant disorder of the oral mucosa; its etiology has not been defined. Our previous study with DNA-binding fluorescent dye suggested the presence of mycoplasmas in the epithelial cells of leukoplakia tissue. Our aim was to detect M. salivarium in the epithelial cells of leukoplakia by immunohistochemistry. We produced a polyclonal antibody (PAb) reactive to Mycoplasma by injecting a rabbit with M. salivarium cells (ATCC 23064) mixed with complete Freund's adjuvant and a monoclonal antibody specific to M. salivarium by injecting M. salivarium cells (ATCC 23557) mixed with complete Freund's adjuvant into the footpads of a rat. Then, we attempted to detect M. salivarium in the epithelium of leukoplakia tissues by immunohistochemistry. We obtained an antimycoplasma rabbit PAb reactive to all seven Mycoplasma species used in this study. Three hybridoma clones producing monoclonal antibodies specific to M. salivarium were obtained, and an M. salivarium-specific monoclonal antibody, designated 7-6H, was established. Immunohistochemistry with these antibodies revealed M. salivarium in the epithelial cells of leukoplakia with hyperplasia and hyperkeratosis on histology. PCR and sequencing verified the presence of M. salivariumDNA in the epithelial cells of leukoplakia. Intracellular M. salivarium was identified in the epithelial cells of leukoplakia.
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影响因子：
11.8
作者：
LO, SC;WEAR, DJ;LEGIER, JF
通讯作者：
LEGIER, JF
影响因子：
4.8
作者：
Mizuki, H
通讯作者：
Mizuki, H
影响因子：
3.1
作者：
Winner, F;Rosengarten, R;Citti, C
通讯作者：
Citti, C
影响因子：
6.1
作者：
Diaz-Garcia, Francisco Javier;Herrera-Mendoza, Alma Patricia;Guerra-Infante, Fernando Martin
通讯作者：
Guerra-Infante, Fernando Martin
DOI：
10.1073/pnas.92.22.10197
发表时间：
1995-10-24
影响因子：
11.1
作者：
TSAI, S;WEAR, DJ;LO, SC
通讯作者：
LO, SC