Prelude to corneal tissue engineering - gaining control of collagen organization.
Prelude to corneal tissue engineering - gaining control of collagen organization.
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DOI：
10.1016/j.preteyeres.2008.08.001
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发表时间：
2008-09
影响因子：
17.8
通讯作者：
Zieske, James D.
中科院分区：
文献类型：
--
作者：
Ruberti, Jeffrey W.;Zieske, James D.
根据大多数标准的工程实践原则，现在讨论人类角膜的“工程”还为时过早。专业的设计工程师会断言，我们仍然不知道角膜是什么（正确地说），因此我们不可能建造一个。证据在于，目前还没有基于经典组织工程方法的临床可行的角膜。这可能是因为传统意义上的组织工程（用合成活性细胞群体接种可降解支架）不产生支持组织化组织产生的条件。解决该问题的替代方法尚处于起步阶段，包括尝试重演发育或从头产生需要最小重塑的角膜基质类似物的方法。尽管如此，组织工程的努力，其已经集中在生产角膜的基本功能成分（胶原或“laminate”的有组织的交替阵列）可能已经提供了有价值的新的见解和工具，与一般结缔组织相关的发育，生长，重塑和病理学。这是因为工程师提出了一个根本不同的问题（如何做到这一点？）生物科学家（如何做到这一点？）探究的差异促使我们仔细检查（和模仿）发育以及研究胶原的物理化学行为，以便我们可以在细胞培养（体外）和实验台上（从头）对组织进行控制。我们的初步结果表明，复制角膜基质样的局部和远程组织的胶原蛋白可能比我们预期的更简单，而控制间距和原纤维形态仍然很困难，但也许不是不可能的（合理）在短期内。
By most standard engineering practice principles, it is premature to credibly discuss the “engineering” of a human cornea. A professional design engineer would assert that we still do not know what a cornea is (and correctly so), therefore we cannot possibly build one. The proof resides in the fact that there are no clinically viable corneas based on classical tissue engineering methods available. This is possibly because tissue engineering in the classical sense (seeding a degradable scaffolding with a population synthetically active cells) does not produce conditions which support the generation of organized tissue. Alternative approaches to the problem are in their infancy and include the methods which attempt to recapitulate development or to produce corneal stromal analogs de novo which require minimal remodeling. Nonetheless, tissue engineering efforts, which have been focused on producing the fundamental functional component of a cornea (organized alternating arrays of collagen or “lamellae”) may have already provided valuable new insights and tools relevant to development, growth, remodeling and pathologies associated with connective tissue in general. This is because engineers ask a fundamentally different question (How can that be done?) than do biological scientists (How is that done?). The difference in inquiry has prompted us to closely examine (and to mimic) development as well as investigate collagen physicochemical behavior so that we may exert control over organization both in cell-culture (in vitro) and on the benchtop (de novo). Our initial results indicate that reproducing corneal stroma-like local and long-range organization of collagen may be simpler than we anticipated while controlling spacing and fibril morphology remains difficult, but perhaps not impossible in the (reasonably) near term.
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影响因子：
5.7
作者：
Aghamohammadzadeh, H;Newton, RH;Meek, KM
通讯作者：
Meek, KM
影响因子：
2.7
作者：
BARD, JBL;ABBOTT, AS
通讯作者：
ABBOTT, AS
影响因子：
1.6
作者：
Brown, CT;Lin, P;Trinkaus-Randall, V
通讯作者：
Trinkaus-Randall, V
DOI：
10.1083/jcb.74.3.816
发表时间：
1977-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bard JB;Higginson K
通讯作者：
Higginson K
影响因子：
14
作者：
Baker, Brendon M.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.