Phylogenetic analyses of an isolate obtained from potato in 1985 revealed potato virus X was introduced to China via multiple events

Phylogenetic analyses of an isolate obtained from potato in 1985 revealed potato virus X was introduced to China via multiple events
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对 1985 年从马铃薯中获得的分离株进行系统发育分析表明,马铃薯 X 病毒通过多个事件传入中国

DOI:
10.1007/s11262-010-0468-5
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发表时间:
2010-03
期刊:
影响因子:
1.6
通讯作者:
Wang, Yu-Jun
Wang, Yu-Jun
中科院分区:
医学4区
文献类型:
--
作者:
Yu, Xiao-Qing;Jia, Jin-Lei;Zhang, Cheng-Ling;Li, Xiang-Dong;Wang, Yu-Jun

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马铃薯X病毒(Potato Virus X,PVX)是茄科植物最常见的病毒之一,给茄科植物造成了巨大的经济损失。我们已经报道了2006年中国马铃薯分离物FX 21的全基因组序列,并证明PVX分离物聚为两个组:欧亚和美洲。在这里,我们提出了完整的基因组序列的一个PVX分离物在1985年从马铃薯(PVX-1985)。PVX-1985的基因组长度与FX 21的基因组长度相同,并且具有相同的基因组结构。PVX-1985与FX 21一样属于欧亚组,与来自欧洲的分离株聚在一起,而FX 21与主要来自亚洲的分离株聚在一起。全基因组序列和CP基因序列的系统发育分析表明,中国PVX分离物具有不同的起源,并通过多个事件引入。虽然PVX的所有开放阅读框都处于负/纯化选择下,但RNA依赖的RNA聚合酶的中心区域处于正/多样化选择下。
Potato virus X (PVX) is one of the most common plant viruses that cause great economic losses to solanaceous plants. We have previously reported the complete genomic sequence of the 2006 Chinese potato isolate FX21 and demonstrated that PVX isolates cluster into two groups: Eurasia and America. Here, we present the complete genomic sequence of one PVX isolate collected from potato in 1985 (PVX-1985). The genome of PVX-1985 is identical to that of FX21 in length and has the same genomic structure. PVX-1985, which like FX21 fell within the Eurasia group, clustered together with isolates from Europe, whereas FX21 clustered together with isolates of primarily Asian origin. Phylogenetic analyses of the complete genomic sequences and of CP gene sequences showed that Chinese PVX isolates have different origins and were introduced via multiple events. Though all the open reading frames of PVX are under negative/purifying selection, the central region of RNA-dependent RNA polymerase is under positive/diversifying selection.
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