Interactions between human milk oligosaccharides, microbiota and immune factors in milk of women with and without mastitis.
Interactions between human milk oligosaccharides, microbiota and immune factors in milk of women with and without mastitis.
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DOI：
10.1038/s41598-022-05250-7
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发表时间：
2022-01-25
影响因子：
4.6
通讯作者：
Fernández L
中科院分区：
文献类型：
--
作者：
Castro I;García-Carral C;Furst A;Khwajazada S;García J;Arroyo R;Ruiz L;Rodríguez JM;Bode L;Fernández L
哺乳期乳腺炎是研究HMO，免疫因子和牛奶微生物群之间可能相互作用的一个很好的目标，因为这种疾病具有感染性和炎症性。在这项工作中，微生物，免疫学和HMO配置文件的牛奶样品的妇女（MW）或没有（HW）乳腺炎进行了比较。女性的分泌状态（基于HMO特征）与乳腺炎无关。分泌型妇女中，HW样本中的DFLNH、LNFP II和LSTb浓度高于MW样本。来自HW的牛奶的特征在于低细菌负荷（主要是表皮葡萄球菌和链球菌）、高IL 10和IL 13流行率以及低唾液酸化HMO浓度。相反，高水平的葡萄球菌、链球菌、IFNγ和IL 12表征来自MW的乳汁。亚急性（SAM）和急性（AM）乳腺炎病例之间的比较显示了与病原体（S。SAM的表皮;乳免疫谱（SAM中高含量的IL 10和IL 13以及AM中高含量的IL 2）和乳HMO谱（SAM中高含量的3FL以及AM中高含量的LNT、LNnT和LSTc）。这些结果表明，牛奶的微生物，免疫学和HMOs谱与妇女的乳房健康有关。
Lactational mastitis is an excellent target to study possible interactions between HMOs, immune factors and milk microbiota due to the infectious and inflammatory nature of this condition. In this work, microbiological, immunological and HMO profiles of milk samples from women with (MW) or without (HW) mastitis were compared. Secretor status in women (based on HMO profile) was not associated to mastitis. DFLNH, LNFP II and LSTb concentrations in milk were higher in samples from HW than from MW among Secretor women. Milk from HW was characterized by a low bacterial load (dominated by Staphylococcus epidermidis and streptococci), high prevalence of IL10 and IL13, and low sialylated HMO concentration. In contrast, high levels of staphylococci, streptococci, IFNγ and IL12 characterized milk from MW. A comparison between subacute (SAM) and acute (AM) mastitis cases revealed differences related to the etiological agent (S. epidermidis in SAM; Staphylococcus aureus in AM), milk immunological profile (high content of IL10 and IL13 in SAM and IL2 in AM) and milk HMOs profile (high content of 3FL in SAM and of LNT, LNnT, and LSTc in AM). These results suggest that microbiological, immunological and HMOs profiles of milk are related to mammary health of women.
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影响因子：
5.6
作者：
Bent R;Moll L;Grabbe S;Bros M
通讯作者：
Bros M
影响因子：
4.2
作者：
Delgado, Susana;Arroyo, Rebeca;Rodriguez, Juan M.
通讯作者：
Rodriguez, Juan M.
影响因子：
5.2
作者：
Sabaté Brescó M;Harris LG;Thompson K;Stanic B;Morgenstern M;O'Mahony L;Richards RG;Moriarty TF
通讯作者：
Moriarty TF
影响因子：
2.5
作者：
Contreras, G. Andres;Miguel Rodriguez, Juan
通讯作者：
Miguel Rodriguez, Juan
影响因子：
4.2
作者：
Goehring, Karen C.;Marriage, Barbara J.;Buck, Rachael H.
通讯作者：
Buck, Rachael H.