Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition.
Hierarchical Action of Mulberry miR156 in the Vegetative Phase Transition.
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DOI：
10.3390/ijms22115550
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发表时间：
2021-05-24
影响因子：
5.6
通讯作者：
He N
中科院分区：
文献类型：
--
作者：
Li H;Luo Y;Ma B;Hu J;Lv Z;Wei W;Hao H;Yuan J;He N
营养阶段的转变是被子植物开花的先决条件。桑树miR156已被证实是拟南芥营养阶段转变的关键因素。miR156在转基因加拿大杨中的过表达显著延长了幼期。我们发现，在桑树的所有组织中，mno-miR156的表达随着年龄的增长而降低，这使得我们研究了miR156在桑树中的分级作用。利用降解组测序和双荧光素酶报告基因分析，9个MnSPLs被证明直接受miR156调控。酵母单杂交和双荧光素酶报告基因检测结果也显示，6个MnSPLs能够识别mno-miR172的启动子序列并激活其表达。我们的研究结果表明，mno-miR156通过抑制桑树MnSPL/mno-miR172通路的表达来发挥其作用。本研究揭示了桑树发育过程中miR156/SPLs/miR172调控通路，填补了我们在多年生木本植物营养相变分子机制方面的空白。
The vegetative phase transition is a prerequisite for flowering in angiosperm plants. Mulberry miR156 has been confirmed to be a crucial factor in the vegetative phase transition in Arabidopsis thaliana. The over-expression of miR156 in transgenic Populus × canadensis dramatically prolongs the juvenile phase. Here, we find that the expression of mno-miR156 decreases with age in all tissues in mulberry, which led us to study the hierarchical action of miR156 in mulberry. Utilizing degradome sequencing and dual-luciferase reporter assays, nine MnSPLs were shown to be directly regulated by miR156. The results of yeast one-hybrid and dual-luciferase reporter assays also revealed that six MnSPLs could recognize the promoter sequences of mno-miR172 and activate its expression. Our results demonstrate that mno-miR156 performs its role by repressing MnSPL/mno-miR172 pathway expression in mulberry. This work uncovered a miR156/SPLs/miR172 regulation pathway in the development of mulberry and fills a gap in our knowledge about the molecular mechanism of vegetative phase transition in perennial woody plants.
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DOI：
10.3390/molecules23010004
发表时间：
2017-12-21
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chen H;Yu W;Chen G;Meng S;Xiang Z;He N
通讯作者：
He N
影响因子：
64.5
作者：
Wu G;Park MY;Conway SR;Wang JW;Weigel D;Poethig RS
通讯作者：
Poethig RS
影响因子：
5.6
作者：
Shalom L;Shlizerman L;Zur N;Doron-Faigenboim A;Blumwald E;Sadka A
通讯作者：
Sadka A
影响因子：
4.8
作者：
Tang, Guiliang;Yan, Jun;Gu, Yiyou;Qiao, Mengmeng;Fan, Ruiwen;Mao, Yiping;Tang, Xiaoqing
通讯作者：
Tang, Xiaoqing
影响因子：
5.1
作者：
Jung, Jae-Hoon;Seo, Pil Joon;Park, Chung-Mo
通讯作者：
Park, Chung-Mo