Molecular epidemiology of Giardia duodenalis infection in humans in Southern Ethiopia: a triosephosphate isomerase gene-targeted analysis.
Molecular epidemiology of Giardia duodenalis infection in humans in Southern Ethiopia: a triosephosphate isomerase gene-targeted analysis.
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DOI：
10.1186/s40249-018-0397-4
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发表时间：
2018-03-05
影响因子：
8.1
通讯作者：
Leyns L
中科院分区：
文献类型：
--
作者：
Damitie M;Mekonnen Z;Getahun T;Santiago D;Leyns L
贾第鞭毛虫是一个由多个遗传上不同的组合组成的物种复合体。该物种给发展中国家带来了重大的公共卫生危机。然而，这些国家的物种的分子多样性、传播动力学和风险因素尚不确定。本研究的目的是确定G.埃塞俄比亚南部无症状个体中的猪链球菌感染。从2014年3月至6月，从590名随机选择的个体中收集了新鲜粪便样本。社会人口数据收集使用预先测试的结构化问卷。采用磷酸丙糖异构酶基因为基础的巢式聚合酶链反应和DNA测序进行基因分型。使用基本的局部比对搜索工具和系统发育分析来确定分离物的遗传同一性和相关性。与G.使用二项和多项逻辑回归模型分析尿道感染。结果表明，18.1%（92/509）的研究对象感染了G。尿道炎其中35.9%（33/92）为A群，21.7%（20/92）为B群，42.4%（39/92）为A群和B群混合感染。绝大多数组合A菌株（94%，31/33）与GenBank登录序列100%同源，其中绝大多数属于亚组合AII。然而，组合B的高遗传变异性和双峰频率使亚基因分型变得更加困难，只有20%（4/20）的分离物与序列100%匹配。年龄（P = 0.032）和饮用水源类型（P = 0.003）与G.尿道感染本研究确定了G.埃塞俄比亚南部的一种寄生虫。A群感染率高于B群，儿童、饮用自来水和露天泉水者感染率高于其他人群。组合B的分型和确定双峰的起源具有挑战性。目前的研究证实，需要进一步的包容性研究进行集中的子类型的组合B和起源的异质性。本文的在线版本（10.1186/s40249-018-0397-4）包含补充材料，可供授权用户使用。
Giardia duodenalis is a species complex consisting of multiple genetically distinct assemblages. The species imposes a major public health crisis on developing countries. However, the molecular diversity, transmission dynamics and risk factors of the species in these countries are indeterminate. This study was conducted to determine the molecular epidemiology of G. duodenalis infection in asymptomatic individuals in Southern Ethiopia. From March to June 2014, fresh stool samples were collected from 590 randomly selected individuals. Socio-demographic data were gathered using a pre-tested structured questionnaire. The genotyping was done using triosephosphate isomerase gene-based nested polymerase chain reaction and DNA sequencing. The genetic identity and relatedness of isolates were determined using the basic local alignment search tool and phylogenetic analysis. Risk factors associated with G. duodenalis infection were analysed using binary and multinomial logistic regression models. The results showed that 18.1% (92/509) of the study subjects were infected by G. duodenalis. Among the isolates, 35.9% (33/92) and 21.7% (20/92) were sub-typed into assemblages A and B, respectively, whereas 42.4% (39/92) showed mixed infections of A and B. Most of the assemblage A isolates (94%,31/33) were 100% identical to sequences registered in GenBank, of which the majority belonged to sub-assemblage AII. However, the high genetic variability and frequency of double peaks made sub-genotyping of assemblage B more problematic and only 20% (4/20) of the isolates matched 100% with the sequences. The risk factors of age (P = 0.032) and type of drinking water source (P = 0.003) both showed a significant association with the occurrence G. duodenalis infection. This study established the endemicity of G. duodenalis in Southern Ethiopia. Infection with assemblage A was more frequent than with assemblage B, and the rate of infection was higher in children and in municipal/tap and open spring water consumers than the other groups. Sub-typing of assemblage B and determining the origin of double peaks were challenging. The present study confirms the need for further inclusive studies to be conducted focusing on sub-types of assemblage B and the origin of heterogeneity. The online version of this article (10.1186/s40249-018-0397-4) contains supplementary material, which is available to authorized users.
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DOI：
10.4269/ajtmh.13-0271
发表时间：
2014-01
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Hong SH;Anu D;Jeong YI;Abmed D;Cho SH;Lee WJ;Lee SE
通讯作者：
Lee SE
影响因子：
4
作者：
Lasek-Nesselquist E;Welch DM;Sogin ML
通讯作者：
Sogin ML
影响因子：
4.2
作者：
Ankarklev J;Svärd SG;Lebbad M
通讯作者：
Lebbad M
影响因子：
3.7
作者：
Anuar TS;Azreen SN;Salleh FM;Moktar N
通讯作者：
Moktar N
影响因子：
3.2
作者：
David ÉB;Guimarães S;de Oliveira AP;Goulart de Oliveira-Sequeira TC;Nogueira Bittencourt G;Moraes Nardi AR;Martins Ribolla PE;Bueno Franco RM;Branco N;Tosini F;Bella A;Pozio E;Cacciò SM
通讯作者：
Cacciò SM