Impact of Phosphorylcholine Expression on the Adherence and Invasion of Streptococcus pyogenes to Epithelial Cells.
Impact of Phosphorylcholine Expression on the Adherence and Invasion of Streptococcus pyogenes to Epithelial Cells.
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DOI：
10.3390/microorganisms10030527
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发表时间：
2022-02-28
期刊：
影响因子：
4.5
通讯作者：
Yamashita M
中科院分区：
文献类型：
--
作者：
Iuchi H;Ohori J;Matsuzaki H;Tokushige T;Toge S;Yamashita M
磷酸胆碱（PC）是多种病原体的结构成分，并通过血小板活化因子受体（PAF-R）参与细菌粘附。在这项研究中，我们研究了PC表达如何影响化脓性链球菌（S。化脓性）。8株临床分离的S.培养化脓性链球菌，并使用荧光激活细胞分选测量PC表达。使用Detroit 562细胞检查细菌粘附和侵袭。用抗PC特异性单克隆抗体TEPC-15抑制细菌PC，用PAF-R拮抗剂ABT-491抑制细胞PAF-R。用标准引物进行聚合酶链反应扩增emm基因。S.化脓菌表面在每个菌株中不同，甚至在相同的EMM基因型中也不同。粘附实验表明TEPC-15和ABT-491的抑制作用与S.化脓同样，细胞内侵袭实验显示TEPC-15和ABT-491的抑制作用与S.化脓本研究表明，S.化脓性链球菌参与PC的细胞粘附和侵袭。
Phosphorylcholine (PC) is a structural component of various pathogens and is involved in bacterial adhesion via the platelet-activating factor receptor (PAF-R). In this study, we investigated how PC expression affects cell adhesion and invasion of Streptococcus pyogenes (S. pyogenes). Eight clinical strains of S. pyogenes were cultured, and PC expression was measured using fluorescence-activated cell sorting. Bacterial adherence and invasion were examined using Detroit 562 cells. An anti-PC-specific monoclonal antibody (TEPC-15) was used to inhibit bacterial PC, and a PAF-R antagonist (ABT-491) was used to inhibit cellular PAF-R. The emm gene was amplified by the polymerase chain reaction with the standard primers. The level of PC expressed on the S. pyogenes surfaces differed in each strain and differed even in the same emm genotype. Adherence assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in S. pyogenes. Similarly, intracellular invasion assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in S. pyogenes. This study suggests that S. pyogenes is involved in cell adhesion and invasion by PC.
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影响因子：
3.7
作者：
Syrogiannopoulos GA;Grivea IN;Al-Lahham A;Panagiotou M;Tsantouli AG;Michoula Ralf René Reinert AN;van der Linden M
通讯作者：
van der Linden M
影响因子：
1.4
作者：
KURONO, Y;SHIGEMI, H;MOGI, G
通讯作者：
MOGI, G
影响因子：
4.5
作者：
Iuchi H;Ohori J;Kyutoku T;Ito K;Kawabata M
通讯作者：
Kawabata M
DOI：
10.1016/j.diagmicrobio.2010.09.011
发表时间：
2011-02-01
影响因子：
2.9
作者：
Chang, Hesheng;Shen, Xuzhuang;Yang, Yonghong
通讯作者：
Yang, Yonghong
影响因子：
3.4
作者：
Molinari, G;Rohde, M;Chhatwal, GS
通讯作者：
Chhatwal, GS