Structural and functional analysis of intra-articular interzone tissue in axolotl salamanders.
Structural and functional analysis of intra-articular interzone tissue in axolotl salamanders.
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DOI：
10.1016/j.joca.2012.07.002
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发表时间：
2012-11
影响因子：
7
通讯作者：
MacLeod, J. N.
中科院分区：
文献类型：
--
作者：
Cosden-Decker, R. S.;Bickett, M. M.;Lattermann, C.;MacLeod, J. N.
指导关节发育的机制可能有助于了解关节病理学和确定新的治疗方法。我们以前已经确定，蝾螈可以完全修复大关节软骨病变，这可能是由于在关节内空间的interzone-like组织的存在。研究的目的是进一步表征蝾螈diarthrodial关节结构，并确定在骨骼微环境中的interzone-like组织的分化潜力。在性成熟（>12个月）的蝾螈的股胫关节中检查了关节形态和聚集蛋白聚糖、BOC、I型胶原、II型胶原和GDF 5的表达。在2-24月龄的个体中评价关节组织细胞构成。通过将中间带样组织放入4 mm胫骨缺损中来评价中间带的软骨形成潜力。在股髋臼和肱桡关节中达到完全空化，但在远端肢体关节的关节内间隙中保留了一个中间区样组织。关节组织细胞减少到7月龄，然后保持稳定。关节标记的基因表达模式在发育中的哺乳动物和成熟的蝾螈中大致相似。当间带样组织移植到临界尺寸的骨骼缺损中时，在缺损部位内形成了副关节。这些实验表明，成熟的蝾螈diarthrodial关节是表型相似的发展中的哺乳动物滑膜关节。由间带样组织产生的副关节表明多能细胞分化潜能类似于哺乳动物胎儿的间带细胞。这些数据支持美西螈作为一种新的脊椎动物模型的联合发展和修复。
Mechanisms directing diarthrodial joint development may be useful in understanding joint pathologies and identifying new therapies. We have previously established that axolotl salamanders can fully repair large articular cartilage lesions, which may be due to the presence of an interzone-like tissue in the intra-articular space. Study objectives were to further characterize axolotl diarthrodial joint structure and determine the differentiation potential of interzone-like tissue in a skeletal microenvironment. Diarthrodial joint morphology and expression of Aggrecan, BOC, Type I Collagen, Type II Collagen, and GDF5 were examined in femorotibial joints of sexually mature (>12 months) axolotls. Joint tissue cellularity was evaluated in individuals from 2-24 months of age. Chondrogenic potential of the interzone was evaluated by placing interzone-like tissue into 4mm tibial defects. Cavitation reached completion in the femoroacetabular and humeroradial joints, but an interzone-like tissue was retained in the intraarticular space of distal limb joints. Joint tissue cellularity decreased to 7 months of age and then remained stable. Gene expression patterns of joint markers are broadly similar in developing mammals and mature axolotls. When interzone-like tissue was transplanted into critical size skeletal defects, an accessory joint developed within the defect site. These experiments indicate that mature axolotl diarthrodial joints are phenotypically similar to developing synovial joints in mammals. Generation of an accessory joint by interzone-like tissue suggests multipotent cellular differentiation potential similar to that of interzone cells in the mammalian fetus. The data support the axolotl as a novel vertebrate model for joint development and repair.
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影响因子：
64.5
作者：
BENYA, PD;PADILLA, SR;NIMNI, ME
通讯作者：
NIMNI, ME
影响因子：
2.5
作者：
Mulieri, PJ;Kang, JS;Krauss, RS
通讯作者：
Krauss, RS
DOI：
10.1097/00003086-198604000-00036
发表时间：
1986-04-01
影响因子：
4.2
作者：
SCHMITZ, JP;HOLLINGER, JO
通讯作者：
HOLLINGER, JO
影响因子：
2.7
作者：
Sobkow, L;Epperlein, HH;Tanaka, EM
通讯作者：
Tanaka, EM
影响因子：
7
作者：
Cosden, R. S.;Lattermann, C.;Romine, S.;Gao, J.;Voss, S. R.;MacLeod, J. N.
通讯作者：
MacLeod, J. N.