Identification and mapping of Epstein-Barr virus early antigens and demonstration of a viral gene activator that functions in trans
Identification and mapping of Epstein-Barr virus early antigens and demonstration of a viral gene activator that functions in trans
复制标题
EB 病毒早期抗原的鉴定和作图以及反式功能的病毒基因激活剂的演示
DOI：
10.1128/jvi.60.1.149-156.1986
复制
发表时间：
1986
影响因子：
5.4
通讯作者：
A. Levine
中科院分区：
文献类型：
--
作者：
K. M. Wong;A. Levine
将eb病毒（Epstein-Barr virus， EBV）基因组BamHI - M DNA片段沿两个方向插入到基于猴病毒40的表达载体中，检测了COS-7猴细胞中产生的EBV特异性蛋白。在一个称为BamHI-M右读框1 （BMRF1）的方向上，合成了一组大小在47,000到54,000道尔顿之间的磷酸化蛋白。这些蛋白与单克隆和多克隆抗血清反应，将其定义为EBV早期抗原弥散蛋白组（EA-D）的组成部分。相反方向的BamHI-M DNA片段，称为BamHI-M左读框1 (BMLF1)，指导了鼻咽癌患者血清抗体检测到的核抗原的合成。针对EA-D复合物的单克隆或多克隆抗体未检测到BMLF1抗原。在BamHI M DNA片段中构建了一系列缺失突变体，并将EA-D复合物和BMLF1抗原映射到该DNA片段的离散开放阅读框中。对这些抗原的几种可能功能的测试表明，BMLF1抗原具有反式激活或增强腺病毒早期3区启动子或猴病毒40早期启动子控制下的基因表达水平的能力，在没有其顺式作用增强子的情况下。这些实验证明了一种新的基因功能，由EBV编码，可能在病毒或细胞基因的积极调节中很重要。
The BamHI M DNA fragment of the Epstein-Barr virus (EBV) genome was inserted in two orientations into a simian virus 40-based expression vector, and the EBV-specific proteins produced in COS-7 monkey cells were examined. In one orientation, termed BamHI-M rightward reading frame 1 (BMRF1), a set of phosphoproteins ranging in size from 47,000 to 54,000 daltons was synthesized. These proteins reacted with monoclonal and polyclonal antisera, defining them as components of the EBV early antigen diffuse set of proteins (EA-D). The BamHI M DNA fragment in the opposite orientation, termed BamHI-M leftward reading frame 1 (BMLF1), directed the synthesis of a nuclear antigen detected by antibodies in serum from a patient with nasopharyngeal carcinoma. The BMLF1 antigen was not detected by monoclonal or polyclonal antibodies directed against the EA-D complex. A series of deletion mutants were constructed in the BamHI M DNA fragment, and the EA-D complex and BMLF1 antigen were mapped to discrete open reading frames in this DNA fragment. A test for several possible functions of these antigens showed that the BMLF1 antigen had the ability to activate or enhance, in trans, the level of expression of a gene under the control of the adenovirus early region 3 promoter or the simian virus 40 early promoter in the absence of its cis-acting enhancer. These experiments demonstrate a new gene function, encoded by EBV, that may be important in the positive regulation of viral or cellular genes.
登录
查看更多内容
用单克隆抗体探测 Epstein-Barr 病毒核抗原的结构。
影响因子：
3.7
作者：
Hearing,JC;Lewis,A;Levine,AJ
通讯作者：
Levine,AJ
DOI：
10.1073/pnas.81.23.7632
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DAMBAUGH, T;HENNESSY, K;KIEFF, E
通讯作者：
KIEFF, E
DOI：
10.1073/pnas.81.12.3806
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YATES, J;WARREN, N;SUGDEN, B
通讯作者：
SUGDEN, B
转移 3.4 兆道尔顿 Epstein-Barr 病毒 DNA 克隆片段后，小鼠细胞中核新抗原稳定表达。
DOI：
10.1073/pnas.79.18.5688
发表时间：
1982
影响因子：
11.1
作者：
Summers,WP;Grogan,EA;Shedd,D;Robert,M;Liu,CR;Miller,G
通讯作者：
Miller,G
Epstein-Barr 病毒 (P3HR-1) 编码早期抗原和病毒衣壳抗原复合物成分的 DNA 存在缺陷。
影响因子：
3.7
作者：
Cho,MS;Gissmann,L;Hayward,SD
通讯作者：
Hayward,SD