DNA barcodes for 1/1000 of the animal kingdom.
DNA barcodes for 1/1000 of the animal kingdom.
复制标题
DOI：
10.1098/rsbl.2009.0848
复制
发表时间：
2010-06-23
期刊：
影响因子：
3.3
通讯作者：
Landry JF
中科院分区：
文献类型：
--
作者：
Hebert PD;Dewaard JR;Landry JF
本研究报告了来自北美东半部的1300多种鳞翅目物种的DNA条形码，确定了其中99.3%的物种具有诊断条形码序列。在这个组合中，种内差异平均只有0.43%，但大多数值更低。在5.1%的物种中，深度条形码差异（大于2%）提高了平均值，通常涉及两个条形码集群的同域发生。对其中一些案例进行了详细分析，揭示了被当前分类系统所忽视的物种。该研究还对条形码序列的区域差异程度进行了大规模测试，表明北美东部鳞翅目的地理差异很小，即使在相距2800公里的种群中进行比较。结果表明，该地区鳞翅目的种内变异有限，单种记录较少，可以建立一个高效的鳞翅目鉴定体系。由于大多数陆地和海洋分类群可能具有相似的种群结构模式，因此可以通过适度的努力开发有效的基于dna的识别系统。
This study reports DNA barcodes for more than 1300 Lepidoptera species from the eastern half of North America, establishing that 99.3 per cent of these species possess diagnostic barcode sequences. Intraspecific divergences averaged just 0.43 per cent among this assemblage, but most values were lower. The mean was elevated by deep barcode divergences (greater than 2%) in 5.1 per cent of the species, often involving the sympatric occurrence of two barcode clusters. A few of these cases have been analysed in detail, revealing species overlooked by the current taxonomic system. This study also provided a large-scale test of the extent of regional divergence in barcode sequences, indicating that geographical differentiation in the Lepidoptera of eastern North America is small, even when comparisons involve populations as much as 2800 km apart. The present results affirm that a highly effective system for the identification of Lepidoptera in this region can be built with few records per species because of the limited intra-specific variation. As most terrestrial and marine taxa are likely to possess a similar pattern of population structure, an effective DNA-based identification system can be developed with modest effort.
登录
查看更多内容
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
估计 DNA 条形码的样本量。
影响因子：
4.1
作者：
Zhang, A. B.;He, L. J.;Zhu, C. -D.
通讯作者：
Zhu, C. -D.
DOI：
10.1073/pnas.0511318103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Smith, MA;Woodley, NE;Hebert, PDN
通讯作者：
Hebert, PDN
影响因子：
1.4
作者：
Briceño, RD;Eberhard, WG;Shelly, TE
通讯作者：
Shelly, TE
DOI：
10.1073/pnas.0510466103
发表时间：
2006-01-24
影响因子：
11.1
作者：
Hajibabaei, M;Janzen, DH;Hebert, PDN
通讯作者：
Hebert, PDN