Stem cell-laden hydrogel bioink for generation of high resolution and fidelity engineered tissues with complex geometries.
Stem cell-laden hydrogel bioink for generation of high resolution and fidelity engineered tissues with complex geometries.
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DOI：
10.1016/j.bioactmat.2021.11.025
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发表时间：
2022-09
影响因子：
18.9
通讯作者：
Alsberg E
中科院分区：
文献类型：
--
作者：
Jeon O;Lee YB;Lee SJ;Guliyeva N;Lee J;Alsberg E
最近，3D生物打印已被探索为生物医学应用的一种有前途的技术，具有创建具有精确特征的复杂结构的潜力。由诸如明胶、胶原、透明质酸、藻酸盐和聚乙二醇的材料组成的细胞包封的水凝胶已被广泛用作用于3D生物打印的生物墨水。然而，由于大多数基于水凝胶的生物墨水可能不允许在3D生物打印后立即快速稳定，因此实现对预期结构的高分辨率和保真度是水凝胶3D生物打印中的常见挑战。在这项研究中，我们利用剪切稀化和自愈离子交联的氧化和甲基丙烯酸化藻酸盐（OMAs）作为生物墨水，其可以在生物打印后通过其自愈性快速凝胶化，并通过二次交联进一步稳定。成功地证明了负载干细胞的钙交联OMA水凝胶可以生物打印成具有高分辨率和保真度的复杂3D组织结构。额外的光交联使得能够长期培养3D生物打印的构建体，以通过包封的人间充质干细胞的分化形成功能组织。具有剪切稀化和快速自修复特性的水凝胶生物墨水系统。生物打印具有高形状保真度和机械稳定性的结构。复杂结构的3D打印，包括精确定义的悬垂几何形状。空间控制组织特异性生物墨水的生物打印的潜力。打印的水凝胶物理和生物化学性质的独立空间控制。
Recently, 3D bioprinting has been explored as a promising technology for biomedical applications with the potential to create complex structures with precise features. Cell encapsulated hydrogels composed of materials such as gelatin, collagen, hyaluronic acid, alginate and polyethylene glycol have been widely used as bioinks for 3D bioprinting. However, since most hydrogel-based bioinks may not allow rapid stabilization immediately after 3D bioprinting, achieving high resolution and fidelity to the intended architecture is a common challenge in 3D bioprinting of hydrogels. In this study, we have utilized shear-thinning and self-healing ionically crosslinked oxidized and methacrylated alginates (OMAs) as a bioink, which can be rapidly gelled by its self-healing property after bioprinting and further stabilized via secondary crosslinking. It was successfully demonstrated that stem cell-laden calcium-crosslinked OMA hydrogels can be bioprinted into complicated 3D tissue structures with both high resolution and fidelity. Additional photocrosslinking enables long-term culture of 3D bioprinted constructs for formation of functional tissue by differentiation of encapsulated human mesenchymal stem cells. Hydrogel bioink system with shear-thinning and rapid self-healing properties. Bioprinting of constructs with high shape fidelity and mechanical stability. 3D printing of complex structures including precisely defined overhang geometries. Potential to spatially control bioprinting of tissue-specific bioinks. Independent spatial control of printed hydrogel physical and biochemical properties.
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影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
DOI：
10.1002/adma.201501099
发表时间：
2015-07-15
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Hong S;Sycks D;Chan HF;Lin S;Lopez GP;Guilak F;Leong KW;Zhao X
通讯作者：
Zhao X
影响因子：
46.9
作者：
Kang, Hyun-Wook;Lee, Sang Jin;Atala, Anthony
通讯作者：
Atala, Anthony
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
14
作者：
Jeon, Oju;Alt, Daniel S.;Ahmed, Shaoly M.;Alsberg, Eben
通讯作者：
Alsberg, Eben