The effect of oxidation on the degradation of photocrosslinkable alginate hydrogels.
The effect of oxidation on the degradation of photocrosslinkable alginate hydrogels.
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DOI：
10.1016/j.biomaterials.2012.01.041
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发表时间：
2012-05
期刊：
影响因子：
14
通讯作者：
Alsberg, Eben
中科院分区：
文献类型：
--
作者：
Jeon, Oju;Alt, Daniel S.;Ahmed, Shaoly M.;Alsberg, Eben
最近，我们报道了一种新的光交联藻酸盐基水凝胶，它具有可控的物理和细胞粘附性能。含有细胞的大分子溶液可以以微创方式注入缺陷部位并交联，同时保持高细胞活力。通过改变藻酸盐聚合物主链上甲基丙烯酸化的程度，可以控制所形成交联中可水解酯键的数目。然而，已经发现水凝胶的降解速度在体内比在体外慢。本研究的目的是开发具有更高生物降解率的光交联海藻酸盐水凝胶，以便在再生医学和生物活性因子递送应用中更快地进行体内生物降解。因此，我们在甲基丙烯酸化之前氧化海藻酸盐，将海藻酸盐残基的构象改变为开链加合物，使其更容易被水解。在这里，我们证明了由氧化的甲基丙烯酸海藻酸盐（OMAs）形成的光交联水凝胶的膨胀行为、降解特征和储存模量可以通过改变海藻酸盐氧化的程度来调节。当与人骨髓间充质干细胞（hBMMSCs）培养时，OMA大分子和光交联OMA水凝胶表现出细胞相容性。此外，来自骨髓或脂肪组织的hMSCs在这些水凝胶中保持活力，其增殖速率是海藻酸盐氧化水平和初始水凝胶重量分数的函数。氧化使光交联OMA水凝胶具有更广泛的物理性质，这可能增强这些治疗材料在组织工程和其他生物医学应用中的效用。
Recently, we reported on a new photocrosslinkable alginate-based hydrogel, which has controllable physical and cell adhesive properties. The macromer solution containing cells can be injected in a minimally invasive manner into a defect site and crosslinked while maintaining high cell viability. The number of hydrolyzable ester bonds in the formed crosslinks may be controlled by altering the degree of methacrylation on the alginate polymer backbone. However, the degradation rate of the hydrogels has been found to be slower in vivo than in vitro. The purpose of this study was to develop photocrosslinked alginate hydrogels with an increased range of biodegradation rates for more rapid in vivo biodegradation in regenerative medicine and bioactive factor delivery applications. Therefore, we oxidized alginate prior to methacrylation to change the uronate residue conformations to an open-chain adduct, which makes it more vulnerable to hydrolysis. Here, we demonstrate that the swelling behavior, degradation profiles, and storage moduli of photocrosslinked hydrogels formed from oxidized, methacrylated alginates (OMAs) are tunable by varying the degree of alginate oxidation. The OMA macromers and photocrosslinked OMA hydrogels exhibited cytocompatibility when cultured with human bone marrow-derived mesenchymal stem cells (hBMMSCs). In addition, hMSCs derived from bone marrow or adipose tissue photoencapsulated within these hydrogels remained viable, and their proliferation rate was a function of alginate oxidation level and initial hydrogel weight fraction. Oxidation permits a wider range of photocrosslinked OMA hydrogels physical properties, which may enhance these therapeutic materials’ utility in tissue engineering and other biomedical applications.
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影响因子：
4.9
作者：
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通讯作者：
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作者：
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作者：
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作者：
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