Dosage-dependent effects of permethrin-treated nets on the behaviour of Anopheles gambiae and the selection of pyrethroid resistance.
Dosage-dependent effects of permethrin-treated nets on the behaviour of Anopheles gambiae and the selection of pyrethroid resistance.
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DOI：
10.1186/1475-2875-3-22
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发表时间：
2004-07-08
期刊：
影响因子：
3
通讯作者：
Guillet P
中科院分区：
文献类型：
--
作者：
Corbel V;Chandre F;Brengues C;Akogbéto M;Lardeux F;Hougard JM;Guillet P
冈比亚按蚊是撒哈拉以南非洲的主要疟疾病媒，其拟除虫菊酯抗药性的演变和传播令人极为关切，因为经拟除虫菊酯处理的蚊帐在世卫组织全球疟疾控制战略中具有重要意义。kdr（主要的拟除虫菊酯抗性机制）对An.人们对冈比亚了解不多。本研究的目的是确定高剂量或低剂量的氯菊酯是否在其抗性选择效应方面存在差异。在实验室条件下，使用隧道试验技术对易感、杂合和纯合基因型进行氯菊酯处理的效果评估。然后在贝宁进行了实验性小屋试验，以评估用各种氯菊酯浓度处理的蚊帐所提供的个人保护水平及其对kdr等位基因选择的影响。隧道试验表明，与较低浓度（100 mg/m2）相比，用250和500 mg/m2氯菊酯处理的蚊帐在易感和杂合（RS）雌性中诱导了更高的死亡率和吸血减少。实验性小屋试验表明，使用最高浓度的氯菊酯（1 000毫克/平方米）可实现最佳的个人保护。mosmetry基因分型显示kdr敏感和耐药基因型的存活率与氯菊酯剂量呈非线性关系。高剂量（≥250 mg/m2）比低剂量（50和100 mg/m2）更有效地杀死RS基因型。这项研究表明，用高浓度氯菊酯处理的蚊帐对拟除虫菊酯抗性的按蚊提供了更好的吸血预防。冈比亚比浓度低。在kdr突变罕见且主要以杂合形式存在的地区，氯菊酯处理的蚊帐似乎不太可能选择拟除虫菊酯抗性。
The evolution and spread of pyrethroid resistance in Anopheles gambiae s.s, the major malaria vector in sub-Saharan Africa, is of great concern owing to the importance of pyrethroid-treated nets in the WHO global strategy for malaria control. The impact of kdr (the main pyrethroid-resistance mechanism) on the behaviour of An. gambiae is not well understood. The objective of this study was to determine whether high or low doses of permethrin differ in their resistance-selection effects. The effect of permethrin treatment was assessed under laboratory conditions using the tunnel test technique against susceptible, heterozygous and homozygous genotypes. Experimental huts trials were then carried out in Benin to assess the level of personal protection conferred by nets treated with a variety of permethrin concentrations and their impact on the selection for kdr allele. Tunnel tests showed that nets treated with permethrin at 250 and 500 mg/m2 induced higher mortality and blood feeding reduction among susceptible and heterozygous (RS) females as compared to the lower concentration (100 mg/m2). The experimental hut trials showed that the best personal protection was achieved with the highest permethrin concentration (1,000 mg/m2). Mosquito genotyping revealed a non-linear relationship in the survival of kdr susceptible and resistant genotypes with permethrin dosage. Higher dosages (≥250 mg/m2) killed more efficiently the RS genotypes than did lower dosages (50 and 100 mg/m2). This study showed that nets treated with high permethrin concentrations provided better blood feeding prevention against pyrethroid-resistant An. gambiae than did lower concentrations. Permethrin-treated nets seem unlikely to select for pyrethroid resistance in areas where the kdr mutation is rare and present mainly in heterozygous form.
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DOI：
10.4269/ajtmh.1995.52.377
发表时间：
1995-05-01
影响因子：
3.3
作者：
CHOI, HW;BREMAN, JG;SEXTON, JD
通讯作者：
SEXTON, JD
影响因子：
2.9
作者：
Corbel, V;Darriet, F;Hougard, JM
通讯作者：
Hougard, JM
DOI：
10.1098/rstb.1998.0329
发表时间：
1998-10-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Curtis, CF;Miller, JE;Kasumba, I
通讯作者：
Kasumba, I
影响因子：
2.9
作者：
Darriet, F;Corbel, V;Hougard, JM
通讯作者：
Hougard, JM
DOI：
10.1002/ps.2780440408
发表时间：
1995-08-01
期刊：
PESTICIDE SCIENCE
影响因子：
--
作者：
ELISSA, N;CURTIS, CF
通讯作者：
CURTIS, CF