Identification of novel QTLs for isolate-specific partial resistance to Plasmodiophora brassicae in Brassica rapa.
Identification of novel QTLs for isolate-specific partial resistance to Plasmodiophora brassicae in Brassica rapa.
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DOI：
10.1371/journal.pone.0085307
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Piao Z
中科院分区：
文献类型：
--
作者：
Chen J;Jing J;Zhan Z;Zhang T;Zhang C;Piao Z
芸苔根肿病（Plasmodiophora brassicae）是芸苔属作物根肿病的病原菌，在世界范围内广泛分布。以大白菜自交系C59-1为感病轮回亲本，芜菁自交系ECD 04为抗病供体亲本，利用甘蓝型油菜两个亚种间杂交的BC 1F 1群体，研究了部分抗性的QTL定位。在受控条件下评估BC 1F 2家系的抗性。利用SSR标记、单基因微卫星标记和特异性标记构建了与已发表的B根肿病抗性基因连锁的遗传图谱。用芜菁进行QTL定位。共检测到6个QTL，分布在B的5个CR QTL区域。芜菁染色体A01，A03和A08被确定为占12.2%至35.2%的表型变异。除Crr 1、Crr 2和Crr 3的3个QTL区域外，还发现了2个新的QTL区域。QTL定位结果表明，1个QTL区域对Pb 2和Pb 7菌株的部分抗性是共有的，而其他QTL区域则对每个菌株都是特异的。此外，还对B. rapa和拟南芥的研究表明，所有CR QTL区域均与3条拟南芥染色体上的单个保守十字花科基因组（U、F和R）对齐，其中在A中检测到2个CR QTL。thaliana.这些结果表明，在B中，一些共同的祖先基因组区域参与了CR基因的进化。拉帕。
Plasmodiophora brassicae, the causal agent of clubroot disease of the Brassica crops, is widespread in the world. Quantitative trait loci (QTLs) for partial resistance to 4 different isolates of P. brassicae (Pb2, Pb4, Pb7, and Pb10) were investigated using a BC1F1 population from a cross between two subspecies of Brassica rapa, i.e. Chinese cabbage inbred line C59-1 as a susceptible recurrent parent and turnip inbred line ECD04 as a resistant donor parent. The BC1F2 families were assessed for resistance under controlled conditions. A linkage map constructed with simple sequence repeats (SSR), unigene-derived microsatellite (UGMS) markers, and specific markers linked to published clubroot resistance (CR) genes of B. rapa was used to perform QTL mapping. A total of 6 QTLs residing in 5 CR QTL regions of the B. rapa chromosomes A01, A03, and A08 were identified to account for 12.2 to 35.2% of the phenotypic variance. Two QTL regions were found to be novel except for 3 QTLs in the respective regions of previously identified Crr1, Crr2, and Crr3. QTL mapping results indicated that 1 QTL region was common for partial resistance to the 2 isolates of Pb2 and Pb7, whereas the others were specific for each isolate. Additionally, synteny analysis between B. rapa and Arabidopsis thaliana revealed that all CR QTL regions were aligned to a single conserved crucifer blocks (U, F, and R) on 3 Arabidopsis chromosomes where 2 CR QTLs were detected in A. thaliana. These results suggest that some common ancestral genomic regions were involved in the evolution of CR genes in B. rapa.
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影响因子：
3.7
作者：
Hatakeyama K;Suwabe K;Tomita RN;Kato T;Nunome T;Fukuoka H;Matsumoto S
通讯作者：
Matsumoto S
影响因子：
3.1
作者：
Li, Xiaonan;Ramchiary, Nirala;Lim, Yong Pyo
通讯作者：
Lim, Yong Pyo
影响因子：
2.7
作者：
CRUTE, IR;PHELPS, K;CRISP, P
通讯作者：
CRISP, P
影响因子：
1.9
作者：
Cowling, W. A.;Buirchell, B. J.;Falk, D. E.
通讯作者：
Falk, D. E.
DOI：
10.2503/jjshs1.77.150
发表时间：
2008-04-01
影响因子：
--
作者：
Hayashida, Nobuaki;Takabatake, Yuri;Matsumoto, Etsuo
通讯作者：
Matsumoto, Etsuo